Objective To investigate the diagnostic value of biochemical test in diabetes.Methods 50 patients with diabetes admitted to Suzhou Wuzhong District Second People's Hospital from June 2021 to June 2023 were selected as the observation group.In the same period,50 healthy objects were treated as control group.Both groups volun-teered to cooperate in the investigation and research,and performed biochemical tests by chemiluminescence immuno-assay.The measures included fasting blood glucose,2 h postprandial blood glucose,insulin level and C-peptide.Blood glucose indicators(including fasting blood glucose and 2 h postprandial blood glucose)were compared between the two groups.Insulin and C-peptide were compared between the two groups(including fasting,1 h,2 h postpran-dial).The diagnostic efficiency of single diagnosis and combined diagnosis of different indexes were compared.Re-sults The fasting blood glucose(8.42±1.02)mmol/L and 2 h postprandial blood glucose(11.16±1.62)mmol/L in the observation group were lower than those in the control group(4.62±0.72)mmol/L and(6.52±0.76)mmol/L,the differ-ences were statistically significant(t=21.521,18.335,both P<0.001).The levelsof insulin and C-peptide at fasting and 2 h after meal in the observation group were higher than those in the control group,while the levels of insulin and C-peptide at 1 h after meal were lower than those in the control group,the differences were statistically significant(all P<0.05).The final clinical diagnosis results showed that 50 cases were positive and 50 cases were negative.The sensi-tivity,accuracy and specificity of combined diagnosis were higher than those of single index diagnosis,and the differ-ences were statistically significant(all P<0.05).Conclusion The diabetes can be evaluated according to the fasting and 2 h postprandial blood glucose and the fasting,1 h,2 h postprandial insulin level and C-peptide level.Mean-while,the combined diagnostic efficacy of these indicators is significantly better than that of a single indicator.