Objective To investigate the role of eukaryotic initiation factor(EIF)4E in ovarian cancer and its effect on the expression of vascular endothelial growth factor(VEGF)A and cyclin D1(CCND1).Methods SKOV3 ovarian can-cer cells were used to analyze the online data to compare the expression differences of EIF4E,VEGFA and CCND1 in ovarian cancer patients and normal sample.The three-dimensional cell culture experiment of EIF4E inhibitor 4EGI-1 was carried out,and the mRNA and protein levels were compared by reverse transcription-polymerase chain reaction(RT-PCR)and Western Blot.Results Cancer genome atlas(TCGA)data showed that the expression levels of VEGFA and CCND1 were significantly increased in ovarian cancer patients,and the differences were statistically significant(both P<0.05).EIF4E was positively correlated with VEGFA and CCND1(both P<0.05).In SKOV3 cells treated with 4EGI-1,the expression of EIF4E,VEGFA and CCND1 were significantly decreased,and the differences were statisti-cally significant(t=9.819,3.508,3.289,all P<0.05).In addition,the expression level of p-EIF4E protein in the 4EGI-1 treatment group was(0.33±0.14)%,which was significantly lower than that in the control group(1.02±0.07)%,and the difference was statistically significant(t=8.818,P=0.0001).At the same time,the levels of VEGFA and CCND1 protein in the 4EGI-1 treatment group were also significantly decreased(t=5.064,6.334,P<0.05).Conclusion EIF4E is highly expressed in ovarian cancer.The EIF4E inhibitor 4EGI-1 May play a role by regulating the expression of VEGFA and CCND1,so EIF4E may be a potential target for ovarian cancer treatment.
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