Inhibition of EIF4E Down-regulates the Expression of VEGFA and CCND1 to Inhibit the Progression of Ovarian Cancer
Objective To investigate the role of eukaryotic initiation factor(EIF)4E in ovarian cancer and its effect on the expression of vascular endothelial growth factor(VEGF)A and cyclin D1(CCND1).Methods SKOV3 ovarian can-cer cells were used to analyze the online data to compare the expression differences of EIF4E,VEGFA and CCND1 in ovarian cancer patients and normal sample.The three-dimensional cell culture experiment of EIF4E inhibitor 4EGI-1 was carried out,and the mRNA and protein levels were compared by reverse transcription-polymerase chain reaction(RT-PCR)and Western Blot.Results Cancer genome atlas(TCGA)data showed that the expression levels of VEGFA and CCND1 were significantly increased in ovarian cancer patients,and the differences were statistically significant(both P<0.05).EIF4E was positively correlated with VEGFA and CCND1(both P<0.05).In SKOV3 cells treated with 4EGI-1,the expression of EIF4E,VEGFA and CCND1 were significantly decreased,and the differences were statisti-cally significant(t=9.819,3.508,3.289,all P<0.05).In addition,the expression level of p-EIF4E protein in the 4EGI-1 treatment group was(0.33±0.14)%,which was significantly lower than that in the control group(1.02±0.07)%,and the difference was statistically significant(t=8.818,P=0.0001).At the same time,the levels of VEGFA and CCND1 protein in the 4EGI-1 treatment group were also significantly decreased(t=5.064,6.334,P<0.05).Conclusion EIF4E is highly expressed in ovarian cancer.The EIF4E inhibitor 4EGI-1 May play a role by regulating the expression of VEGFA and CCND1,so EIF4E may be a potential target for ovarian cancer treatment.
万方数据
维普
