Analysis of the Results of Identification of Non-tuberculous Mycobacterio-sis Species Based on Next Generation Sequencing Technology
Objective The identification results of nontuberculous mycobacteria(NTM)by next generation sequencing(NGS)were analyzed to provide a basis for the diagnosis of NTM disease.Methods A total of 91 patients'sample with NTM admitted to the First Affiliated Hospital of Guangxi Medical University from January 2020 to October 2023 were selected as the research objects,and the results of strain identification were analyzed based on NGS detection technol-ogy.Results Among the 91 patients,46 were diagnosed with NTM lung disease and 45 were diagnosed with dissemi-nated NTM disease.The type of specimens submitted by NTM lung disease patients was mainly alveolar lavage fluid(95.7%),and a small part was lung tissue(4.3%).The types of specimens submitted by patients with disseminated NTM lung disease were diverse,mainly lymph node tissue(35.6%)and alveolar lavage fluid(26.7%),followed by skin tissue(8.9%).The age of patients with NTM lung disease was significantly higher than that of patients with dissemi-nated NTM disease,and the difference was statistically significant(P<0.05).There was no significant difference in gender distribution between the two groups(P>0.05).In the NTM lung disease group,mycobacterium avium complex(54.4%)was the most common,followed by mycobacterium intracellulare(26.1%),followed by mycobacterium chelo-nei(8.7%).The distribution of species in the disseminated NTM disease group was similar.Mycobacterium avium complex(53.3%)was the most common,followed by mycobacterium abscessus(26.7%),mycobacterium fortuitum(8.9%),and others were relatively rare.Conclusion NTM disease in Guangxi is widely distributed,with slow-growing NTM common,and involves a variety of specimen types,should be suspected patients as early as possible for mycobac-terial identification,NGS can be used as a rapid means of detection,to provide a basis for clinical diagnosis.
万方数据
维普
