Effect of Sevoflurane on Invasion Ability of Glioma U87 Cells and Its Mechanism
Objective To explore the impact of sevoflurane on U87 cells migration.Methods U87 cells,which pro-vided by anesthesiology Laboratory of Xuzhou Medical University were cultured to logarithmic growth stage and then randomly random grouping for all experiments.In Transwell experiment,cells were divided into 8 parts,and then ran-domly divided into control(Con)and sevoflurane(Sev)groups(with 4 parts in each group for parallel experiments)to detect the impact of sevoflurane on the migration between September 2022 and June 2023.Cultured cells were placed in an anaesthetic chamber 1.4%sevoflurane with carrier gas in the sevoflurane group and carrier gas only in the con-trol group for 4 h.The Transwel assay was used to evaluate the migration of U87 cells;In WB experiment,cells were divided into 12 parts,and then randomly divided into control group,sevoflurane 2 h group and sevoflurane 4 h group(with 4 parts in each group for parallel experiments)to evaluate the level of pERK1/2 and ERK1/2 expression;In im-munofluorescence experiment,cells at logarithmic growth stage were divided into 8 parts,and then randomly divided into control and sevoflurane groups(4 h)(with 4 parts in each group for parallel experiments)to detect pERK1/2 and Nrf2 cell distribution.Results Compared with the control group,the number of U87 cells passing through the micropo-rous membrane in sevoflurane group decreased[Con group(100.000±0.000)%,Sev group(34.180±3.342)%],and the difference was statistically significant(t=16.697,P＜0.05).Compared with the control group,the gray value of pERK1/2 in sevoflurane group decreased[Con group(100.000±0.000)%,Sev2 h group(0.196±0.028)%,Sev4 h group(0.073±0.025)%],and the difference was statistically significant(F=546.886,P＜0.05).The expression levels of pERK1/2[Con group(21.903±1.776)%,Sev group(8.412±0.367)%]and Nrf2 were significantly decreased[Con group(18.083±0.788)%,Sev group(12.315±1.604)%],the differences were statistically significant(t=7.438,3.228,both P＜0.05).Conclusion Sevoflurane inhibits the invasion of human glioma U87 by inhibiting ERK1/2-Nrf2 signal pathway.
SevofluraneExtracellular signal-regulated kinaseTranscription factor NF-E2 related factor 2Human glioblastoma
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