Effect of NT3 Gene Transfection on Noise Induced Hearing Loss
Objective To verify the protective effect of cationic liposomes mediated neurotrophin-3(NT3)gene trans-fection on noise induced hearing loss in guinea pigs.Methods 60 healthy black eye guinea pigs of different colors were selected as the study objects from the Animal Experiment Center of Guangxi Medical University and were ran-domly divided into 5 groups by random number table method,with 12 animals in each group(some animals died dur-ing the experiment,and the actual experimental data was calculated based on 10 animals in each group).Group I was not affected by noise,Group Ⅱ was not stimulated by noise after injection of artificial external lymph,Group Ⅲ was stimulated by noise after injection of artificial external lymph,Group Ⅳ was stimulated by noise after injection of empty plasmid,and Group V was stimulated by noise after injection of plasmid containing NT3 gene.Steady state white noise with a noise intensity of 4 kHz,100 dB,8 h/d,continuous for 10 days,resting for 5 days.Then,auditory brainstem response(ABR)testing and immunohistochemical staining were used to evaluate changes in hearing thresh-old and expression of NT3 protein in cochlear spiral ganglion cells.Results The ABR threshold range of Group V was higher than Group I and Group Ⅱ,but lower than Group Ⅲ and Group Ⅳ,and the differences were statistically sig-nificant(all P<0.05).The expression level of NT3 protein in the spiral ganglion cells of the cochlea in Group V was higher than those in the other groups,and the differences were statistically significant(all P<0.05).Conclusion After NT3 gene transfection,the threshold increase decreases,and the impairment of hearing function is alleviated to a cer-tain extent,which has a certain degree of protective effect on noise induced deafness in guinea pigs.
万方数据
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