The Protective Mechanism of Resveratrol in Alleviating Secondary Renal Injury After Ureteral Obstruction by Regulating the AREG/EGFR Path-way
Objective To explore the protective mechanism of resveratrol(RSV)in alleviating secondary renal injury after ureteral obstruction through the amphiregulin(AREG)/epidermal growth factor receptor(EGFR)pathway.Meth-ods Thirty SD rats raised in the Experimental Animal Center of Mudanjiang Medical College from February to Septem-ber 2023 were selected as the research objects and divided into control group according to the random number table method,unilateral ureteral obstruction(UUO)group and UUO+resveratrol group,with 10 rats in each group.The mRNA expression levels of malondialdehyde(MDA),superoxide dismutase(SOD),AREG and EGFR in the three groups were compared.The markers of AREG/EGFR pathway and related indicators of renal interstitial fibrosis(RIF)[fibronectin(FNO),α-smooth muscle actin,renal interstitial fibrosis(RIF)]were analyzed.α-SMA),E-cadherin].Re-sults Compared with the control group,the protein expression levels of α-SMA and FNO in UUO group were in-creased,the protein expression of E-cadherin was decreased,the MDA expression level was increased,the SOD ex-pression level was decreased,and the protein expressions of AREG and EGFR were increased,the differences were statistically significant(all P<0.05).Compared with UUO group,α-SMA and FNO protein expression decreased,E-cadherin protein expression increased,MDA expression level decreased,SOD expression level increased,and AREG and EGFR protein expression decreased in UUO+RSV group,with statistical significance(all P<0.05).Conclusion Resveratrol can improve RIF and oxidative stress in UUO rats by regulating AREG/EGFR pathway.
AREG/EGFR signal communicationResveratrolUnilateral ureteral obstruction in ratsRenal fibrosisoxidative stress
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