Repair Effect of Mouse Bone Marrow Mesenchymal Stem Cells on Acute Liver Injury in Mice
Objective To investigate the restorative effect of mouse bone marrow mesenchymal stem cells(BM-MSCs)on mouse acute liver injury induced by tetrachloromethane(CCl4).Methods The study was carried out in Jiangsu Prov-ince Hospital of Chinese Medicine through out the year 2018.Eighteen mice were divided into three groups by random number table method,namely mineral oil group,CCl4-injuried group and BM-MSCs-treated group.Each group,con-taining six mice,respectively received a single dose of mineral oil or 10%CCl4 by intraperitoneal injection to establish acute liver injury model,followed by intravenous administration of phosphate buffered saline(PBS)or BM-MSCs 24 hours later.In the 4th day,blood samples from mice were collected to detect the activities of alanine aminotransferase(AST)and alanine aminotransferase(ALT)in serum.Liver tissues were prepared to evaluate changes in appearance and histopathology.Results The serum levels of ALT(67.40±5.79)U/L and AST(110.70±39.58)U/L in CCl4 group were significantly higher than those of ALT(51.53±9.52)U/L and AST(73.65±9.77)U/L in Oil group.While BM-MSCs administration reduced both to lower levels,namely ALT(54.18±17.19)U/L and AST(78.73±14.97)U/L,com-pared to CCl4 group,the differences were statistically significant(all P<0.05).In terms of changes in hepatic appear-ance and pathological structure,typical oxidative damage was observed in CCl4 group,which was relieved to a signifi-cant extent after BM-MSCs treatment.Conclusion Administration of exogenous mouse BM-MSCs may contribute to restoration of mouse acute liver injury induced by CCl4.
Bone marrow mesenchymal stem cellsTetrachloromethaneAcute liver injury
万方数据
维普
