Objective To explore the effect of arbutin on mouse oocyte maturation and early embryonic development in vitro.Methods Oocytes from mice at the germinal vesicle(GV)stage were cultured in vitro for 16 h using medium containing different doses of arbutin(0,10,20,and 40 μmol/L,set up as control,10 μM,20 μM,and 40 μM groups,respectively).Oocyte maturation rate and reactive oxygen species(ROS)levels were monitored.After in vitro fertiliza-tion,fertilized eggs were transferred to the same medium and changes in cleavage rate and blastocyst rate were ob-served.Results There was no statistically significant difference in the number of oocytes among the four groups(all P>0.05).The in vitro oocyte maturation of oocytes supplemented with arbutin medium was higher than that of control oo-cytes,and the 40μM group(100%)was significantly higher than the control group(50%),the difference was statisti-cally significant(χ2=10.526,P=0.001).There was no statistically significant difference in the first polar body dis-charge rate among the 4 groups(all P>0.05).The ROS fluorescence intensity in oocytes of 10 μM group,20 μM group and 40 μM group was lower than that of the control group,and the difference was statistically significant(all P<0.05).Conclusion The addition of arbutin in maturation fluid plays an important role in reducing intracellular stress,protect-ing cells from apoptosis and increasing the quality of egg cell maturation,and can reduce the ROS level in oocytes,but has no significant effect on mouse embryo development.
关键词
小鼠/体外成熟/氧化应激/熊果苷
Key words
Mouse/Maturation in vitro/Oxidative stress/Arbutin
维普
万方数据