Mechanism of Effect of Arbutin on Mouse Oocyte Maturation and Early Embryonic Development Potential in Vitro
Objective To explore the effect of arbutin on mouse oocyte maturation and early embryonic development in vitro.Methods Oocytes from mice at the germinal vesicle(GV)stage were cultured in vitro for 16 h using medium containing different doses of arbutin(0,10,20,and 40 μmol/L,set up as control,10 μM,20 μM,and 40 μM groups,respectively).Oocyte maturation rate and reactive oxygen species(ROS)levels were monitored.After in vitro fertiliza-tion,fertilized eggs were transferred to the same medium and changes in cleavage rate and blastocyst rate were ob-served.Results There was no statistically significant difference in the number of oocytes among the four groups(all P>0.05).The in vitro oocyte maturation of oocytes supplemented with arbutin medium was higher than that of control oo-cytes,and the 40μM group(100%)was significantly higher than the control group(50%),the difference was statisti-cally significant(χ2=10.526,P=0.001).There was no statistically significant difference in the first polar body dis-charge rate among the 4 groups(all P>0.05).The ROS fluorescence intensity in oocytes of 10 μM group,20 μM group and 40 μM group was lower than that of the control group,and the difference was statistically significant(all P<0.05).Conclusion The addition of arbutin in maturation fluid plays an important role in reducing intracellular stress,protect-ing cells from apoptosis and increasing the quality of egg cell maturation,and can reduce the ROS level in oocytes,but has no significant effect on mouse embryo development.
万方数据
维普
