NF-κB p65 Improves Renal Ischemia-reperfusion Injury Through the miR-150/TRPC6 Signaling Pathway
Objective Exploring the role of nuclear transcription factor-κB(NF-κB)p65 in promoting renal ischemia-reperfusion injury through the miR-150/transient receptor potential cation channel 6(TRPC6)signaling pathway.Methods A total of sixty male wistar rats purchased by the Research Institute of Medicine and Pharmacy of Qiqihar Medical College from August 2022 to August 2023 were selected as research subjects.Rat with similar body weight were randomly divided into three groups using a random number table method:sham surgery experimental group(Sham group,n=10),ischemia/in focusischemic/reperfusion(I/R)experimental group(n=10),and lentivirus ex-perimental group(n=40).The lentivirus experimental group was divided into miR-150-mimic group(n=10),I/R+miR-150-mimic group(n=10),NF-κB p65-si group(n=10),and I/R+NF-κB p65-si group(n=10).Evaluate the levels of serum creatinine(Cr),blood urea nitrogen(BUN),inflammatory factors,miR-150,NF κB p65,and TRPC6 in the re-nal tissues of rats in each group.Results Compared with the Sham group,the I/R experimental group showed a de-crease in NF-κB p65 and miR-150 protein levels(0.26±0.02 vs 0.79±0.03,0.34±0.08 vs 1.46±0.12),and an de-crease in TRPC6 protein levels(0.35±0.04 vs 1.08±0.15),the differences were statistically significant differences(t=46.484,24.558,8.603,all P<0.05).Compared with the I/R experimental group,the level of TRPC6 in the I/R+miR-150-mimic group increased,while the level of inflammatory factors decreased.The level of miR-150 in the I/R+NF-κB p65-si group was higher,and the level of inflammatory factors increased.The differences were statistically significant(all P<0.05).Conclusion NF-κB p65 affects renal function and inflammation by regulating the miR-150/TRPC6 signaling pathway,and improves renal ischemia-reperfusion injury.
NF-κB p65miR-150Transition receptor potential cation channel subfamily C member 6Renal ischemia-reperfusion injury
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