Comparison of the Diagnostic Efficacy of Chemiluminescence Immunoas-say and Enzyme-linked Immunosorbent Assay for Hepatitis B
Objective To compare and analyze the diagnostic efficacy of chemiluminescence immunoassay and enzyme-linked immunosorbent assay in the diagnosis of viral hepatitis B.Methods The purpose sampling method was used to select two hundred and twenty suspected hepatitis B patients admitted to Gaomi People's Hospital from Janu-ary 2022 to August 2023 as the research subjects.Both chemiluminescence and enzyme-linked immunosorbent assay(ELISA)methods were used for detection,and the results of fluorescence quantitative polymerase chain reaction PCR(qPCR)were used as the gold standard to compare the diagnostic efficacy and serological positivity rates of the two methods.Results The qPCR results showed that one nundred and thirty-three cases were positive and eighty seven cases were negative.There was no statistically significant difference(P>0.05)in the accuracy,sensitivity,and specific-ity of chemiluminescence immunoassay compared to enzyme-linked immunosorbent assay.The positive rates of HB-sAg,HBeAg and HBeAb in chemiluminescence immunoassay were 97.74% (130/133),96.99% (129/133)and 97.74% (130/133),respectively,which were higher than 78.95% (105/133),78.95% (105/133)and 91.95% (109/133)in enzyme-linked immunosorbent assay,and the differences were statistically significant(χ2=22.821,20.462,18.179,all P<0.05).Conclusion Chemiluminescence immunoassay usually has significant advantages in sensitivity,specificity,and automation,especially suitable for large-scale detection and high-throughput laboratories.
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