Electroacupuncture Combined with Rehabilitation Training Mediates Ln-cRNA MALAT1/BDNF Regulation of Cortical Synaptic Plasticity in Mice with Cerebral Ischemia Reperfusion Injury
Objective To explore the mechanism of electroacupuncture combined with rehabilitation training on im-proving cortical synaptic plasticity in mice with cerebral ischemia-reperfusion injury(CIRI).Methods Thirty SPF C57BL/6 mice purchased from the Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine from January to June 2024 were selected as research objects and divided into sham group,MCAO/R(M)group,ER-M group,sh-NC-M group,sh-MALAT1-M group and sh-MALAT1-M-ER group according to random number table,with 5 mice in each group.The modified neurological severity score(mNSS)scale was used to evaluate the neurologi-cal function of mice 1 day after the completion of model creation and 7 days of treatment.The cerebral infarction vol-ume of mice was measured.the synaptic ultrastructures of mice were observed under transmission electron micro-scope.Western blot was used to analyze the expression of brain-derived neurotrophic factor(BDNF),postsynaptic den-sity 95(PSD95),and synapsin-1 protein in brain tissue.The RNA expression levels of long non-coding RNA metasta-sis associated lung adenocarcinoma transcript 1(LncRNA MALAT1),BDNF,PSD95 and synapsin-1 were detected by qRT-PCR.Pearson analyzed the correlation between LncRNA MALAT1 and BDNF mRNA levels.RNA-binding pro-tein immunoprecipitation analyzed the interaction of LncRNA MALAT1 and BDNF.Results On the first day after modeling,the mNSS scores of mice in other groups were significantly higher than those in Sham group,and the differ-ences were statistically significant(all P<0.05).Compared with the Sham group,the mNSS score,cerebral infarction volume,and LncRNA MALAT1 level on the 7th day of treatment in Group M and sh-NC-M group were significantly increased,and the ER-M group was significantly lower than that in Group M,the sh-MALAT1-M group was signifi-cantly lower than that in Group sh-NC-M,and the sh-MALAT1-M-ER group was significantly lower than that in ER-M and sh-MALAT1-M groups,and the differences were statistically significant(all P<0.05).The number of syn-apses,BDNF,PSD95,synapsin-1 protein and mRNA expression in M and sh-NC-M groups were significantly lower than those in Sham group,the ER-M group was significantly higher than that in M group,the sh-MALAT1-M group was significantly higher than that in sh-NC-M group,and the sh-MALAT1-M-ER group was significantly higher than that in ER-M and sh-MALAT1-M groups,and the differences were statistically significant(all P<0.05).There was a significant correlation between LncRNA MALAT1 level and BDNF mRNA level(r=-0.751,P<0.001).Com-pared with the control group using immunoglobulin G for co-immunoprecipitation,the expression level of LncRNA MALAT1 in the BDNF protein-RNA complex was significantly increased,and the differences were statistically signifi-cant(P<0.05).Conclusion Electroacupuncture combined with rehabilitation training can promote the recovery of neu-ral function in CIRI mice by mediating LncRNA MALAT1/BDNF,reduce cerebral infarction volume,and improve syn-aptic plasticity in cerebral cortex.
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