Objective To detect the expression level of C1q tumor necrosis factor-related protein 2(CTRP2)in the aorta of patients,aorta of mouse and mouse aortic vascular smooth muscle cells,and to analyze its correlation with the pathogenesis of aortic dissection(AD).Methods The GEO database analyzed the difference in expression of CTRP2 in patients with and without AD.The aortic specimens of patients diagnosed with acute Stanford type A AD in Renmin Hospital of Wuhan University from June 2021 to December 2022 were selected as the dissection group(n=6),and the aorta of heart transplant patients was used as the control group(n=6),and the difference in CTRP2 expression between the dissection group and the control group was detected by Western blot method.Twenty-four C57BL/6 male 3-week-old wild-type mice were randomly classified into control group and dissection group,and the dissection group was fed with feed containing 0.25％β-aminopropionitrile for 4 weeks.The aorta tissue was embedded with paraffin,and the specimens were sectioned and detected the difference in CTRP2 expression by immunohistochemistry.The cells were divided into control group and dissection group,and mouse aortic vascular smooth muscle cells were extracted after treating with PBS and different concentrations of angiotensin Ⅱ.The expression level of CTRP2 was detected by Western blot method.Results The results of immunohistochemistry and Western blot showed that the expression of CTRP2 in the aorta of patients,aorta of mouse and mouse aortic vascular smooth muscle cells in the dissected group were significantly increased.Conclusion There are differences in the expression level of CTRP2 protein in the control group and the dissection group,which is correlated with the occurrence of AD,and is expected to become a new therapeutic target for AD.
Aortic dissectionC1q tumor necrosis factor-related protein 2Therapeutic target
维普
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