Objective The occurrence of ventricular arrhythmias has been associated with the β1-adrenergic receptor autoantibodies(β1AAbs).The aim of this study was to investigate the role of β1AAbs on the electrophysiological properties of rat ventricular space and its intervention.Methods Thirty male SD rats aged 6 to 8 weeks(weighing 180 to 220 g)were randomly divided into 3 groups(n=10):control group,β1AAbs group and bisoprolol group.Active immunisation ventricular arrhythmia susceptibility model was established by multi-point injection of β1-adrenergic receptor second extracellular loop antigenic peptide via the back at 0,2,4 and 6 weeks.Serum β1 AAbs levels at different time points were measured to validate the model.The electrophysiological parameters ventricular effective refractory period,effective refractory period dispersion,conduction velocity and conduction heterogeneity were measured in different regions of the ventricle.Masson staining was used to detect fibrosis levels in different regions of ventricular tissue.Results The level of β1AAbs increased significantly from week 2 to week 8 in the β1AAbs group and the bisoprolol group compared with the control group(P＜0.05).Compared with the control and bisoprolol groups,the β1 AAbs group showed a significant increase in heart rate and a significant shortening of RR,QT and QTc intervals(P＜0.05);a significant decrease in ventricular effective refractory period in different regions,a significant increase in effective refractory period dispersion(P＜0.05),a decrease in conduction velocity in different regions and an increase in conduction heterogeneity(P＜0.05),and a significant increase in the percentage of collagen volume in different sites(P＜0.05),and the above parameter changes were most pronounced in the middle section,all of which could be reversed by bisoprolol(P＜0.05).Conclusion β1 AAbs can increase the electrophysiological properties of ventricular space changes,the underlying mechanism may be related to the degree of fibrosis in different regions,and bisoprolol has potential therapeutic value.
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