老年脑出血患者血清lncRNA H19和miR-140-5p表达水平与认知功能障碍的相关性
Correlation among serum lncRNA H19,miR-140-5p expression levels and cognitive dysfunction in elderly patients with cerebral hemorrhage
金铁 1丁继 1崔凯 1李敏1
作者信息
- 1. 国药葛洲坝中心医院重症医学科,湖北宜昌 443000
- 折叠
摘要
目的:探讨老年脑出血患者血清长链非编码RNA H19(lncRNA H19)、微小RNA-140-5p(miR-140-5p)表达水平及与认知功能障碍的相关性.方法:选择2020年1月～2022年1月国药葛洲坝中心医院收治的131例老年脑出血患者,根据简易精神状态检查量表(MMSE)评分将患者分为认知功能正常组(79例,MMSE评分≥26分),认知功能障碍组(52例,MMSE＜26分);另选择同期健康体检者60例作为对照组.收集各组一般资料,检测血清lncRNA H19、miR-140-5p 表达水平;采用 Pearson 法分析 MMSE 评分、血清 lncRNA H19、miR-140-5p 表达水平之间的相关性,多因素Logistic回归模型分析老年脑出血患者发生认知功能障碍的危险因素.结果:与认知功能正常组比较,认知功能障碍组患者美国国立卫生研究院卒中量表(NIHSS)评分及血清lncRNA H19表达水平显著升高,MMSE评分及miR-140-5p表达水平均显著降低(P均＜0.001).在认知功能障碍患者中,MMSE评分与血清lncRNA H19表达水平呈负相关(r=-0.643,P＜0.001),与miR-140-5p表达水平呈正相关(r=0.612,P＜0.001),lncRNA H19表达水平与miR-140-5p表达水平呈负相关(r=-0.561,P＜0.001).多因素Logistic回归分析显示,lncRNAH19是老年脑出血患者发生认知功能障碍的独立危险因素(OR=2.576,P=0.002),而miR-140-5p为独立保护因素(OR=0.659,P=0.038).结论:血清lncRNA H19和miR-140-5p表达水平与老年脑出血患者发生认知功能障碍密切相关,检测其水平对此类患者临床评估具有重要意义.
Abstract
Objective:To investigate serum long non-coding RNA H19(lncRNA H19),microRNA-140-5p(miR-140-5p)expression levels in elderly patients with cerebral hemorrhage,and their correlation with cognitive dysfunction.Methods:A total of 131 elderly patients with cerebral hemorrhage who were treated in Gezhouba Central Hospital of Sinop-harm between January 2020 and January 2022 were selected.According to score of Mini-Mental State Examination(MMSE),the patients were divided into normal cognitive function group(n=79,MMSE score≥ 26 points)and cognitive dysfunction group(n=52,MMSE score＜26 points);another 60 cases undergoing physical examination simultaneously were regarded as the control group.General data were collected in all groups,the serum expression levels of lncRNA H19 and miR-140-5p were measured;the correlation among MMSE score,serum expression levels of lncRNA H19 and miR-140-5p was analyzed by Pearson method;multivariate Logistic regression model was used to analyze risk factors of cog-nitive dysfunction in elderly patients with cerebral hemorrhage.Results:Compared with the normal cognitive function group,patients in cognitive dysfunction group had significant higher score of National Institutes of Health Stroke Scale(NIHSS)and serum lncRNA H19 expression,and significant lower MMSE score and miR-140-5p expression(P＜0.001 all).In patients with cognitive dysfunction,MMSE score was inversely correlated with serum lncRNA H19 expression level(r=-0.643,P＜0.001),and positively correlated with miR-140-5p expression level(r=0.612,P＜0.001),and ln-cRNA H19 expression was inversely correlated with miR-140-5p expression(r=-0.561,P＜0.001).Multivariate Lo-gistic regression analysis indicated that lncRNA H19 was independent risk factor for cognitive dysfunction in elderly patients with cerebral hemorrhage(OR=2.576,P=0.002),while miR-140-5p was its independent protective factor(OR=0.659,P=0.038).Conclusion:Serum lncRNA H19 and miR-140-5p expression levels are closely associated with cog-nitive dysfunction in elderly patients with cerebral hemorrhage,and their detection may have important significance for clinical assessment of these patients.
关键词
脑出血/认知障碍/RNA,未翻译/微RNAsKey words
Cerebral hemorrhage/Cognition disorders/RNA,Untranslated/MicroRNAs引用本文复制引用
出版年
2024
NETL
NSTL
万方数据