Isolation and Screening of Ranunculus ternatus Thunb Botrytis Cinerea Pathogen Antagonistic Bacteria
In order to screen out the antagonistic bacteria against botrytis cinerea of Ranunculus ternatus Thunb,the bacteria were isolated from rhizosphere soil of different plants,and their antagonistic effects on botrytis cinerea were determined by plate confrontation method.The results show that 106 strains of bacteria were isolated and purified,and 13 strains had antago-nistic effect on botridomyces cinerea after preliminary screening.Their numbers are BDYM-1,BDYM-2,BDHS-3,DFYM-1,SQBC-1,SQTS-2,SQBC-8,XJMH-1,NYHS-3,ZKFQ-1,ZKFQ-2,ZKFQ-3,and ZKFQ-6.Af-ter re-screening,5 strains of antagonistic bacteria were selected on the basis of preliminary screening,which were BDYM-1,BDHS-3,SQTS-2,SQBC-8 and ZKFQ-3.The bacteriostatic effect of SQBC-8 was the best,the bacteriostatic rate was 64.4％,and the bacteriostatic effect of BDHS-3 was relatively stable.Preliminary identification was made by morphological characteristics observation,and the identification result was that all 5 strains were Gram-positive.
ranunculus ternatus thunb botrytis cinereaantagonistic bacteriaisolation and screeninggram-positive bacterium
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维普
