从全国各地收集的自然发酵红曲米中分离纯化获386株红曲霉菌株;经筛选获1株高产淀粉酶的Mp‐42优质菌株,根据Mp‐42菌株的形态、生理生化和ITS基因序列,鉴定为紫色红曲霉(Monascus purpureus).Mp‐42菌株具有高产淀粉酶、生长速度快、产孢量多等优良特性,在发酵培养基初始p H为6.0,培养温度为30℃条件下,M p‐42菌株淀粉酶酶活可达168178 U/m L .
On the Screening of One Monascus Strain with High-yielding Amylase and its Culture Characteristics
To obtain Monascus strain which had high efficient degradation for starch ,386 Monascus strains were separated and purified from the natural fermentation of red yeast rice all over the country . Strain Mp‐42 which had high yield of amylase was obtained through screening .According to the morpholo‐gies ,physiological and biochemical characteristics and ITS gene sequences ,strain Mp‐42 was identified as Monascus purpureus .Strain Mp‐42 has many good qualities ,such as high yield amylase ,fast growth ,high amount of spores .Strain Mp‐42 of amylase enzyme activity is up to 168 U/mL -178 U/mL under the con‐dition of the initial fermentation pH 6 .0 ,temperature of 30 ℃ .