Study on the Quality Standard of Tibetan Medicine Ranunculus tanguticus(Maxim.)Ovcz.
Objective To revise and improve the quality standards of Tibetan Medicine Ranunculus tanguticus(Max-im.)Ovcz.to provide a scientific and reliable evaluation basis for the quality control of Ranunculus tanguticus(Max-im.)Ovcz..Methods Fifteen batches of Ranunculus tanguticus(Maxim.)Ovcz.from different sources were subject-ed to character,microscopic,and thin layer identification,moisture,ash,and acid insoluble ash examinaiton,and pre-liminary analysis of leachables and chemical compositions.In this experiment,quercetin was used as a reference for the identificaiton of thin layers of Ranunculus tanguticus(Maxim.)Ovcz..Results The microscopic characteristics of Ranunculus tanguticus(Maxim.)Ovcz.were obvious.Fifteen batches of Ranunculus tanguticus(Maxim.)Ovcz.were tested,and the thin-layer chromatograms of the samples were consistent with the chromatograms of the querce-tin reference substance,showing fluorescent spots of the same color.The moisture,total ash,acid-intolerant ash,and alcohol-soluble extracts of Ranunculus tanguticus(Maxim.)Ovcz.ranged from 8.18%～11.01%,5.6%～7.8%,0.69%～1.20%and 35.02%～39.04%,respectively.The preliminary analysis of the chemical composition of this variety was carried out,and the peaks with the same retention time as the quercetin control and kaempferide control were detected in the 15 batches of samples.Conclusion This study is the first time to comprehensively investigate the quality con-trol indexes of Ranunculus tanguticus(Maxim.)Ovcz.,which can effectively evaluate the quality of Ranunculus tang-uticus(Maxim.)Ovcz.and provide a reference for the quality control of the medicinal material.
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