In order to establish a rapid propagation system for the tissue culture of Motuo wild Anoectochilus rox-burghii,the research selected wild Anoectochilus roxburghii plants collected from Gelin Village,Motuo County,Ny-ingchi Prefecture,Xizang Autonomous Region to conduct the corresponding experiment.The results showed that among the three selected materials,leaf blocks were not suitalbe as explants,and the stem segments and the roots with nodes were suitable as explants,while the optimal disinfection time of HgCl2 solution was 10 minutes.The opti-mal induction medium for callus and adventitious buds is A5 treatment group(MS+0.1g/L inositol+30g/L sucrose+5g/L agar powder+1mg/L IBA+0.5mg/L NAA+2mg/L 6-BA),the healing rate was 73.33%,the bud proliferation factor was 8.87,and the average bud length was 13.30mm.The optimal rooting medium for rootless seedlings was B7 treat-ment group(1/2MS+0.1g/L inositol+30g/L sucrose+5g/L agar powder+0.5mg/L NAA+1mg/L IBA),with an average root number of 4.20 and an average root length of 26.43mm.
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