不同基因型甘薯品种胚性愈伤诱导和植株再生试验及福薯604胚性悬浮体系的建立
Embryogenic callus induction and plant regeneration of different genotypes of sweetpotato varieties and establishment of embryogenic suspension system of Fushu 604
许泳清 1张鸿 1李国良 1李华伟 1林赵淼 1邱永祥 1邱思鑫1
作者信息
- 1. 福建省农业科学院作物研究所/农业农村部南方薯类科学观测实验站,福建福州 350013
- 折叠
摘要
以福薯604、福菜薯18号、福薯7-6等16个甘薯品种为材料,通过茎尖诱导胚性愈伤,进行愈伤分化再生试验,比较不同基因型品种的愈伤诱导率、愈伤生长状态和植株再生率等的差异,结果表明,胚性细胞的诱导率和再生率因品种的不同而呈现明显差异.试验筛选出诱导率高、胚性愈伤状态好、分化再生率高的品种福薯604,并建立其胚性悬浮体系,测量胚性悬浮细胞的生长量,为甘薯优良品种福薯604遗传转化提供技术支持.
Abstract
Sixteen sweetpotato varieties,including Fushu 604,Fucai Potato 18,Fushu 7-6 etc.,were used as mate-rials to induce embryogenic calluses from stem tips and perform callus differentiation and regeneration experiments.The differences in callus induction rate,callus growth status,and plant regeneration rate among different genotypes were compared.The results showed significant differences in induction and regeneration rates of embryogenic cells among varieties.The variety of Fushu 604,which had high induction rates,good embryogenic callus status,and high differentiation regeneration rates,was selected by experiments.An embryogenic suspension system was estab-lished for this variety,and the growth of embryogenic suspension cells was measured to provide technical support for the genetic transformation of the elite sweetpotato variety Fushu 604.
关键词
甘薯/胚性悬浮体系/植株再生Key words
sweetpotato/embryogenic suspension system/plant regeneration引用本文复制引用
基金项目
福建省属公益类科研院所基本科研专项(2021R10310011)
福建省人民政府-中国农业科学院农业高质量发展超越"5511"协同创新工程项目(KXXYJBG0039)
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-10-B14)
出版年
2024
NETL
NSTL
万方数据