Gene cloning and expression analysis of IbCHLH in sweetpotato
CHLH is a multifunctional protein crucial for chlorophyll synthesis and abscisic acid(ABA)signaling.In this article,the IbCHLH gene was cloned from Xushu 29,a bioinformatics analysis was conducted,and the expres-sion of the gene under abiotic stress conditions and exogenous hormone treatments were detected by employing q-PCR.The cloned CDS sequence of IbCHLH had a length of 4 146 bp,which could encode 1 381 amino acids,and a molecular mass of 153.53 kDa.IbCHLH protein was a hydrophilic protein without transmembrane structure.Since the protein has the largest number of Ser phosphorylation sites,it is speculated that its activity was under phospho-rylation mediated regulation.Phylogenetic analysis revealed that the IbCHLH of sweetpotato was most closely relat-ed to the CHLH protein from I.trifida,followed by I.triloba.IbCHLH expression peaked at 24 h under drought stress and at 4 h following ABA treatment within a 24 h period.In conclusion,the IbCHLH gene may engage in drought resistance regulation and ABA regulation.
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