Objective To analyze the composition and drug resistance of pathogens causing bloodstream infection in the hospital in Tibet from 2018 to 2021,and to provide scientific basis for the empirical treatment of bloodstream infection in the region.Meth-ods The positive blood culture samples of hospitalized patients in a hospital during 4 years were retrospectively analyzed,and the composition characteristics of pathogenic bacteria and drug sensitivity results were summarized.The data were statistically analyzed by WHONET 5.6 software and SPSS software.Results A total of 751 strains of pathogenic bacteria were isolated from the blood cul-ture specimens of the patients during the 4 years(excluding the repeated detection of pathogens from the same specimen type of the same patient).Among them,396(52.7%)were Gram-positive bacteria,343(45.7%)were Gram-negative bacteria,and 12(1.6%)were fungi.Conclusions The bloodstream infections in this hospital from 2018 to 2021 are mainly Gram-positive bacteria.The resis-tance of main pathogens to commonly used antibiotics is also on the rise.It is necessary to strengthen the monitoring of the distribu-tion and drug resistance of common bacteria in bloodstream infection,and use targeted antibiotics more rationally.