干细胞来源外泌体治疗大鼠心肌梗死有效性的Meta分析
Meta analysis of efficacy of stem cell derived exosomes in treatment of myocardial infarction in rats
贺天文 1舒兵 2万菁菁1
作者信息
- 1. 武汉大学中南医院心内科,湖北武汉 430071
- 2. 武汉大学中南医院神经外科,湖北武汉 430071
- 折叠
摘要
目的 系统性评价干细胞来源外泌体治疗大鼠心肌梗死的有效性.方法 检索PubMed和中国生物医学文献服务系统(SinoMed)等数据库,采用主题词方式进行检索,外文数据库以Pubmed为例,检索策略为:exosomes AND stem cells AND myocardial infarction;中文数据库以中国生物医学文献服务系统(SinoMed)为例,检索策略为:((外泌体)AND(干细胞))AND(心肌梗死);收集干细胞来源外泌体治疗大鼠心肌梗死模型的相关文献并最终纳入 10篇文献进行Meta分析.首先行异质性检验,然后逐次剔除单个研究,进行敏感性分析,最后漏斗图检测文章发表偏倚.结果 最终纳入 10篇动物实验文献,共 179只大鼠.实验组 97只采用干细胞来源的外泌体治疗,对照组 82只采取空白对照、生理盐水或者PBS干预.Meta分析结果示:干细胞来源的外泌体治疗可以显著提高心肌梗死大鼠的左室射血分数(SMD=13.91,95%CI:8.90～18.91,P<0.01)、左室短轴收缩率(SMD=7.52,95%CI:5.29～9.76,P<0.01)以及减少心梗面积(SMD=-15.15,95%CI:-17.90～-12.40,P<0.01).结论 干细胞来源的外泌体治疗可明显改善心肌梗死大鼠的心功能.
Abstract
AIM To systematically evaluate the effectiveness of stem cell derived exosomes in the treatment of animal models of myocardial infarction.METHODS Using the Pubmed and SinoMed as examples respectively for the databases in English or Chinese,and Medical Subject Heading(MeSH)'s strategy used for the search was(exosomes)AND(stem cells)AND(myocardial infarction)respectively in English and Chinese.The databases of PubMed and SinoMed were searched by computer to collect the relevant literature on the treatment of rat myocardial infarction model with stem cell derived exosomes.Finally,10 articles were included for Meta-analysis.First,the heterogeneity test was performed,then the sensitivity analysis was performed by eliminating the methods of individual studies one by one,and finally the publication bias was detected by funnel chart.RESULTS A total of 10 animal experimental literatures were included,with 179 rats.Of the 179 rats,97 rats in the exogenous group received cell transplantation treatment and 82 rats in the control group received blank control,normal saline or PBS treatment.The results of Meta-analysis showed that Exosomes'therapy significantly increased left ventricular ejection fraction(SMD=13.91,95%CI:8.90～18.91,P<0.01),left ventricular short axis contraction rate(SMD=7.52,95%CI:5.29～9.76,P<0.01)and reduced myocardial infarction area(SMD=-15.15,95%CI:-17.90～-12.40,P<0.01)in rats with myocardial infarction.CONCLUSION The results show that the treatment of stem cell derived exosomes could significantly improve the cardiac functions after myocardial infarction in rats.
关键词
干细胞/心肌梗死/外泌体/大鼠/Meta分析Key words
stem cells/myocardial infarction/exosomes/rats/Meta analysis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据