Preparation of cobalt-doped hydroxyapatite and its osteogenic induction properties in vitro
To optimize the bone-inducing ability of hydroxyapatite(HAp)in the treatment of bone defects,a series of cobalt-doped hydroxyapatite(Co-HAp)with different Co/Ca molar ratios were prepared by chemical precipita-tion method,its morphology and structure were characterized,and its osteogenic induction ability was studied in vitro.The results showed that the synthesized Co-HAp had a typical apatite structure.When the molar ratio of Co/Ca was 0.1%,the crystallinity of Co-HAp was the lowest.When the molar ratio of Co/Ca was 0.1%and the concentration of the extract was 12.5 μg/mL,Co-HAp had the best proliferation ability and good cytocompatibility.At this concentration,Co-HAp could enhance the ALP activity of BMSCs and increase the number of calcium nodules,and show better osteogenic induction ability in vitro.This study can provide theoretical basis for exploring more abundant repair materials in the field of bone defect repair.
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