Adsorption and migration of non-motile Rhodococcus sp.OS62-1 in alfalfa roots
To explore the adsorption and migration of non-motile Rhodococcus sp.OS62-1 with alfalfa roots.The adsorption of OS62-1 on alfalfa roots was observed and counted by scanning electron microscopy and gradient dilu-tion plate method.The migration of OS62-1 in different soils and combined remediation systems was studied by gradient dilution plate method.The residual oil concentration of petroleum contaminated soil at different depths was measured by infrared oil meter,and the remediation effect of different remediation methods at different depths was explored.The results showed that strain OS62-1 was able to effectively adsorb to the alfalfa root system,and the number of OS62-1 cells adsorbed to alfalfa roots was approximately(7.7±1.0)×103 CFU/mm2(colony forming units).The migration ability of strain OS62-1 in uncontaminated soil was much higher than that in petroleum contaminated soil.On the 7th day,OS62-1 had migrated to the depth of 6 cm in uncontaminated soil,while it only migrated to the depth of 2 cm in petroleum contaminated soil.Strain OS62-1 was detected in the rhizosphere of alfalfa in contaminated soil,but OS62-1 was not detected at the depth not reached by the roots.After 11 days repair,the residual oil concentration of oil-contaminated soil treated by single inoculation of OS62-1 and combined remediation was significantly lower than that in the control group,and the residual oil concentration of soil at all depths in the treatment group was lower than that in the control group.The non-motile Rhodococcus sp.OS62-1 can adsorb and migrate to alfalfa root system,thus improving the efficiency of oil pollution remediation in the combined remediation system.
万方数据
维普
