Study on the bacteriostatic activity and mechanism of the adventitious roots extract of Actinidia arguta Sieb.et Zucc.against Bacillus subtilis
In order to explore the practical application of Actinidia arguta Sieb.et Zucc.adventitious roots,the study investigated the bacteriostatic activity of methanol extract from Actinidia arguta Sieb.et Zucc.adventitious roots against Bacillus subtilis and its mechanism.The results showed that the extracts had good inhibitory effect on Bacillus subtilis,with a 21.33 mm inhibitory circle diameter and a minimum inhibitory concentration of 4 mg·mL-1.The extracellular alkaline phosphatase,nucleic acids,proteins and electrolyte levels of Bacillus subtilis treated by the extract were significantly increased,indicating that the extract destroyed the cell membrane and caused the leakage of intracellular substances.Scanning electron microscopy results showed that the cell membrane of Bacillus subtilis treated by the extract was wrinkled and secretions were accumulated.The extract inhibited the respiration of Bacillus subtilis,and the respiratory superposition rate of the extract and sodium phosphate(25.0％)was small,supposing that the extract might inhibit bacterial respiration through the regulation of hexose monophosphate pathway(HMP).Furthermore,the activity of glucose-6-phosphate dehydrogenase(G6PD),one of the key enzymes in HMP,was detected,and it was found that the activity of G6PD was decreased after the treatment of the extract,which proved that the extract was involved in the regulation of HMP.The results of this study can provide reference for the use of Actinidia arguta Sieb.et Zucc.adventitious roots to produce the bacteriostatic products in the future.
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