Analysis of HCV molecular transmission clusters in HIV-HCV co-infected patients in high prevalence area of HIV in Butuo County
Objective To investigate the distribution of subtypes of HCV(hepatitis C virus)strains in HIV(human immunodeficiency virus)-HCVco-infected people population in Temuli Town,Muer Township and Jiudu Township(later called"two townships and one town")in Butuo County,Liangshan Prefecture,to analyze the local epidemiological characteristics,transmission rules and relevant influencing factors of HCV,and to provide scientific basis for the formulation of targeted prevention and control measures of HCV.Methods Plasma from HIV-HCV co-infected people in the two townships and one town of Butuo County from from 2017 to 2020 was collected.The sequence of NS5B region of HCV Gene was obtained according to the operation procedure of genotype detection.The phylogenetic tree was constructed by comparing those gene sequences,and the molecular propagation network was constructed followed by the gene distance method.Chi-square test was used to analyze the distribution of HCV,and multivariate logistic regression model was used to analyze the influencing factors of edge number ≥2 in the propagation network.Results A total of 617 gene fragments in NS5B region of HIV-HCV antibody positive samples were sequenced and tested,and 324 sequences(52.51％)were successfully obtained.Among them,6u,3a,3b and other subtypes were 117 cases(36.11％),87 cases(26.85％),55 cases(16.98％)and 65 cases(20.06％),respectively.At a gene distance of 0.015,the largest number of clusters(20)was observed.A total of 132(40.74％)sequences entered the network after the construction of HCV molecular network.Multivariate logistic regression analysis showed that when the influencing factors of edge number ≥2 in the molecular propagation clusters were studied,the risk of people of other occupations was 0.34 times that of farmers(95％confidence interval CI:0.13-0.90);the risk of CD4+T lymphocytes 200-300 cells/uL before HIV treatment was 2.4 times that of CD4+T lymphocytes ≤200 cells/uL(95％CI:1.08-5.34);the infection rates of 3b subtype and other subtypes were 0.221 times(95％CI:0.09-0.56)and 0.428 times(95％CI:0.20-0.91)those of 6u subtype,respectively.Conclusions The HCV genetypes in Yi nationality area of Liangshan Prefecture were complex and diverse,and the long-term and dynamic monitoring of 6u subtypes and farmers should be strengthened,and effective intervention actions should be taken to ensure accurate disease prevention and control.
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