2017-2022年北京市丰台区食源性疾病沙门菌病原学分析
Pathogenic analysis of salmonella from foodborne diseases in Fengtai District of Beijing from 2017 to 2022
王兆娥 1张志敏 1余红 1曹佳琪 1刘晓玲 1封会茹1
作者信息
- 1. 北京市丰台区疾病预防控制中心,北京 100070
- 折叠
摘要
目的 了解北京市丰台区食源性疾病监测中检出沙门菌的血清型、分子分型及耐药情况.方法 对2017-2022年北京市丰台区3家哨点医院收集的腹泻病例粪便标本中检出的77株沙门菌进行血清分型、分子分型及药物敏感实验,同时对3者间的相关性进行分析.计数资料的比较采用x2检验,以P＜0.05为差异有统计学意义.结果 肠炎沙门菌为优势血清型;74株沙门菌经PFGE聚类分析得到48种带型,各带型条带差异较大,肠炎沙门菌形成2个大簇;77株沙门菌多重耐药率63.64％,耐药率前3位的抗生素为:萘啶酸(67.53％)、链霉素(59.74％)、氨苄西林(57.14％),多重耐药谱有32种,耐药菌株数最多的耐药谱为AMP-AMS-NAL-Sul-CT-STR,且全部为肠炎沙门菌.结论 北京市丰台区沙门菌血清型相同的菌株间PFGE带型一致性较高,成簇现象明显;沙门菌耐药情况严重,耐药谱复杂;血清型与耐药谱间有一定关联性.
Abstract
Objective To investigate the serotype,molecular typing and drug resistance of Salmonella detected in food-borne disease surveillance in Fengtai district of Beijing.Methods Serotyping,molecular typing and drug sensitivity tests were performed on 77 Salmonella strains detected from fecal samples collected from three sentinel hospitals in Fengtai District of Beijing during 2017 to 2022,and their correlations were analyzed,counting data were tested by x2.Results Salmonella enteritidis was the dominant serotype.Forty-eight types of bands were obtained by PFGE cluster analysis of 74 strains of Salmonella.The bands varied greatly,and Salmonella enteritidis formed two large clusters.The multiple drug resistance rate of 77 Salmonella strains was 63.64％,and the top three antibiotics with the drug resistance rate were:Nalidixic acid(67.53％),streptomycin(59.74％),ampicillin(57.14％),there were 32 kinds of multi-drug resistance spectrum,the resistance spectrum of the most resistant strains was AMP-AMS-NAL-Sul-CT-STR,and all were Salmonella enteritidis.Conclusions The PFGE band type of Salmonella strains with the same serotype in our region is consistent and the clustering is obvious.Salmonella resistance is serious and its drug spectrum is complex.There is a certain correlation between serum type,PFGE type and drug resistance spectrum.
关键词
沙门菌/血清分型/分子分型/聚类分析/耐药性分析Key words
salmonella/serotyping/molecular typing/cluster analysis/drug resistance analysis引用本文复制引用
出版年
2024
NETL
NSTL
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