2012-2021年重庆市长寿区外环境禽流感病毒监测结果分析
Surveillance results of avian influenza virus in external environment in Changshou District of Chongqing from 2012 to 2021
邹海桃 1张鹏 1白银 1雷群建1
作者信息
- 1. 重庆市长寿区疾病预防控制中心,重庆 401220
- 折叠
摘要
目的 了解2012-2021年重庆市长寿区外环境禽流感病毒阳性及分布情况,为防控辖区人感染禽流感疫情提供数据支撑.方法 采集2012-2021年重庆市长寿区外环境4种场所的5种类型外环境标本,采用荧光定量PCR方法对标本进行流感病毒甲型通用、H5、H7和H9亚型流感病毒检测.率的比较采用x2检验,P＜0.05为差异有统计学意义.结果 2012-2021年重庆市长寿区采集禽流感外环境标本1074份,检出甲型流感病毒阳性标本202份,阳性率为18.81％,各年度均有阳性标本检出,阳性率差异无统计学意义(x2=6.835,P=0.650);阳性样本中H5亚型占30.69％,H9亚型占51.98％、H5+H9混合型占13.37％,其他型别(甲型非H5、非H9、非H7型别)占3.96％;4类场所中仅城乡活禽市场中检出阳性,阳性率为29.28％;5种类型外环境标本阳性率差异有统计学意义(x2=89.561,P＜0.001),其中清洗禽类污水禽流感病毒检出阳性率最高(35.09％),其次为宰杀或摆放禽肉案板表面擦拭(30.20％);不同季度采集的标本均可检测到阳性,各季度阳性率差异有统计学意义(x2=13.312,P＜0.001),第3季度标本阳性率最高(25.00％),其次为第1季度(20.24％).结论 现有监测数据分析表明:重庆市长寿区活禽市场外环境有长期被甲型流感病毒污染的风险,应警惕活禽市场外环境长期阳性引起禽流感病毒传人的情况发生.
Abstract
Objective To investigate the distribution of avian influenza virus in external environment in Changshou District of Chongqing from 2012 to 2021,and to provide evidence for prevention and control of human infection with avian influenza.Methods From 2012 to 2021,five types of samples were collected from 4 sites in the outer environment in Changshou District.The samples were detected by fluorescent quantitative PCR for influenza virus A,H5,H7 and H9 subtypes.Chi-squre test was used for ratio comparision,with the test leve α=0.05.Results From 2012 to 2021,there were 1 074 avian influenza samples collected from outside environment,and 202 were positive for influenza A virus,with a positive rate of 18.81％.No statistically significant difference was observed in the positive rate between the years(x2=6.835,P=0.650).H5 subtype accounted for 30.69％of the positive samples,H9 subtype accounted for 51.98％,and H5+H9 mixed type accounted for 13.37％of the positive samples.Among the four sites,only urban and rural live poultry markets were positive,the positive rate was 29.28％.The positive rate of the five types of environmental samples was statistically significant(x2=89.561,P＜0.001);the positive rate of avian influenza virus was the highest(35.09％)in washing poultry sewage,followed by wiping the surface of slaughtering or placing poultry meat board(30.20％).There was statistically significance of positive rate in different quarters(x2=13.312,P＜0.001).The highest positive rate was 25.00％in the third quarter,followed by 20.24％in the first quarter.Conclusions The existing monitoring data showed that the environment outside in the live poultry market was contaminated by influenza A virus for a long time in Changshou District.It is necessary to be vigilant about the possibility of human transmission of avian influenza virus caused by positive environment outside the live poultry market.
关键词
禽流感病毒/外环境/监测Key words
avian influenza virus/external environment/surveillance引用本文复制引用
出版年
2024
NETL
NSTL
万方数据