Determination and analysis of the complete genome sequence of a strain of human rabies virus caused by wild boar in Sichuan Province
Objective To determine the whole genome sequence and analyze the phylogenetic and molecular characteristics of a strain of rabies virus infecting humans caused by wild boar,and to understand the molecular epidemiology of rabies virus carried by wild animals in Sichuan Province.Methods The cDNA(complementary deoxyribonucleic acid)was synthesized by reverse transcription PCR(polymerase chain reaction)after the extraction of viral nucleic acid from saliva specimen of a rabies case collected in 2020,and then the whole genome sequence of the virus was amplified and sequenced with specific primers.BioEdit 7.0 software was used for the alignment analysis of the whole genome nucleotide and deduced amino acid sequences,and Mega 7.0 software was used for phylogenetic analysis and phylogenetic tree mapping.Results A complete genome sequence of rabies virus(SCR20-093)with a total length of 11 881 bp was obtained.Phylogenetic and homology analysis showed that SCR20-093 belonged to China Ⅰ.The N gene homology between SCR20-093 and the predominant strains presently in circulation in China ranged from 85.2％to 99.9％and 92.6％to 99.7％,in terms of nucleotide and amino acid homology,respectively.The strains of the Sichuan rabies virus(SCR13-309)and SCR20-093 had the highest nucleotide and amino acid homology and the closest evolutionary relationship.When compared to important domestic and foreign vaccine strains,it was most closely related to Chinese vaccine strain CTN-1 and had the highest homology at the whole genome level.Analysis of important functional sites showed that,when compared with the vaccine strain,the major functional sites of SCR20-093 were relatively conserved,but there were antigenic site differences in N and G genes.Conclusions The complete genome sequence of a strain of human rabies virus caused by wild boar in Sichuan Province was obtained and identified as China Ⅰ,which was consistent with the typing of the perennial rabies virus strain in Sichuan Province.Compared with the vaccine strain,some antigenic sites of SCR20-093 were different,but the main virulence and antigenicity remained unchanged.
万方数据
维普
