预防医学情报杂志2024,Vol.40Issue(8) :1031-1034,1042.DOI:10.19971/j.cnki.1006-4028.240249

石墨炉原子吸收标准加入校正法测定血中铅

Determination of lead in blood by graphite furnace atomic absorption standard addition correction method

陶红霞 卢俊霖 张家树 潘泓宇 钱炳富 邓航 张大芬 谢若愚
预防医学情报杂志2024,Vol.40Issue(8) :1031-1034,1042.DOI:10.19971/j.cnki.1006-4028.240249

石墨炉原子吸收标准加入校正法测定血中铅

Determination of lead in blood by graphite furnace atomic absorption standard addition correction method

陶红霞 1卢俊霖 1张家树 1潘泓宇 1钱炳富 1邓航 1张大芬 1谢若愚1
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作者信息

  • 1. 攀枝花市疾病预防控制中心,四川 攀枝花 617000
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摘要

目的 采用石墨炉原子吸收标准加入校正法即优化原子吸收石墨炉测定血液中铅的方法,为改进检测方法提供参考.方法 血液通过一定比例的硝酸、曲拉通稀释,用1%磷酸二氢铵作基体改进剂,牛血做本底,使用标准加入校正法测定标准曲线后再进行样品的定量测定.所得数据采用Excel 2019软件进行配对z检验,检验水准为0.05.结果 本方法对血铅的测定在0~500μg/L范围里吸光度和铅含量成正比、线性良好相关系数R2=0.998检出限0.67 pg/L、精密度0.8%~3.15%、回收率97%~104%都达到要求.结论 本法全面优化血中铅的测定条件,简单、高效准确,为血中铅测定较为理想的测定方法,可用于普通人群和接触人群中血铅的检测与评价.

Abstract

Objective To optimize the determination of lead in blood by graphite furnace atomic absorption standard addition correction method,and to provide reference for improving the detection method.Methods Blood was diluted with a certain proportion of nitric acid and triton,and 1%ammonium dihydrogen phosphate was used as the base modifier and bovine blood was used as the background.The standard curve was determined by the standard addition correction method,and then the sample was quantitatively determined.The obtained data were examined by paired t-test by Excel 2019.And the test level was a=0.05.Results In the range of 0-50 ug/L,the absorbance of blood lead was proportional to the lead content by this method.With a linear good correlation coefficient of R2=0.998,the detection limit was 0.67ug/L,the precision was 0.8%-3.15%,and the recovery rate was 97%-104%,all meeting the requirements.Conclusions This method potimized the conditions for the determination of lead in blood,is simple,efficient and accurate,and is an ideal method for the determination of lead in blood.It can be used for the detection and evaluation of lead in blood in general population and contact population.

关键词

血铅/标准加入校正法/自动进样/原子吸收

Key words

blood lead/standard addition correction method/automatic sampling/atomic absorption

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基金项目

攀枝花市科技计划项目(2023ZD-S-39)

出版年

2024
预防医学情报杂志
中华预防医学会,四川省疾病预防控制中心

预防医学情报杂志

CSTPCD
影响因子:0.681
ISSN:1006-4028
参考文献量14
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