Study on pre-treatment methods for the determination of 21 elements in serum
Objective The contents of 21 elements in serum was determined by inductively coupled plasma mass spectrometry,and the sample pre-treatment method for multi-element determination in serum was optimized.Methods The serum samples were diluted 20 times with 1％nitric acid,1％TItonX-100,0.01％ammonium pyrrole dithiocarbamate and 1％ethanol.The content of 21 elements such as Mn,Co,Ni,Cd,La,Ce,W,Hg,Tl,Mo,Sb,Fe,Cu,Zn,Se,Al,As,Sr,Pb,Sn and Cr was detected by the ICP-MS instrument.Results The 21 elements in serum had good linearity in the linear range(r＞0.995),and the detection limits ranged from 0.14 to 15.90 μg/L.The method was used for the detection of serum samples from pregnant women.The recovery rates of serum spiked at two levels of low and high concentrations were within 82.2.0％-121.5％,with a relative standard deviation of less than 10％.The serum samples of 639 pregnant women were collected for detection.Among the 21 elements,except for Tl,W,La,Ce,and Hg,all other elements were detected.Conclusions By optimizing the conditions,the mixed solvent dilution method combined with ICP-MS was used to analyze 21 metal elements in serum.The method is fast,simple,and accurate,it provids data reference and technical support for evaluating human element exposure levels.
万方数据
维普
