Investigation and analysis on current situation of nucleic acid detection laboratory capacity building in Qingdao city disease control institution,Shandong provide
Objective To investigate and analyze the status of nucleic acid detection laboratory capacity building of dis-ease control institutions in Qingdao district(city),so as to provide a reference for policy formulation to promote the sus-tainable development of CDC.Methods Questionnaire survey,on-site assessment and personnel interview were used to collect and analyze the data on the establishment of nucleic acid detection functional laboratory,nucleic acid detection per-sonnel,nucleic acid detection equipment,nucleic acid detection capacity,laboratory information construction and qualifica-tion archival status of 10 disease control institutions selected from district(city)of Qingdao,and the data were analyzed.Results The total area of fixed microbiology laboratory in Qingdao district(city)disease control institution was 6 686 square meters,the highest was 2 000 square meters,the mini-mum was 160 square meters;Theve were 30 biosafety level Ⅱlaboratories cgeneral and enhaneds,incluching 14 fixed quantitative polymerase chain reaction(PCR)laboratories and 9 wobik PCR laboratories(including vehicle-mourted,square-cabin and air-film models).There were 106 nucleic acid testing personnel,and the average service resident population was 0.12 people/10 000 people.The education level of the personnel was 91.5％with bachelor degree or above;11.3％with master degree or above,including 1 person with doctoral de-gree.In terms of professional titles,12.3％of personnel had senior professional titles,16.0％had intermediate profession-al titles,and 60.4％had junior professional titles.Among them,5 districts(cities)had no employees with senior profes-sional titles.The male to female ratio was 28:78,1 of the districts(cities)was entirely female,and 55.7％of the popula-tion was under the age of 30.All districts(cities)were equipped with instruments and equipment according to the demand for novel coronavirus pneumonia nucleic acid testing,with a total of 120 fluorescence PCR machines and 66 automatic nu-cleic acid extraction machines.There were 4 districts(cities)equipped with 20 cup dispensing systems,and 2 districts(cit-ies)equipped with 6 pipetting workstations and 17 cup dispensing systems.The laboratories in Qingdao district(city)on-ly carried out 4 types of nucleic acid testing programmes,and all districts(cities)carried out nucleic acid testing for novel coronavirus and norovirus,6 districts(cities)carried out nucleic acid testing for influenza virus,and 3 districts(cities)carried out nucleic acid testing for enterovirus.Laboratory testing information systems were estabilshed in 8 districts(cit-ies),the results of the satisfaction survey showed that 2 were satisfied with the information system,5 were basically satis-fied,and 1 was not satisfied.There were 6 districts(cities)disease prevention and control centre to obtain a valid qualifica-tion certification of Shandong province inspection and testing institutions,none of them have obtained the qualification of PCR laboratory filing in Shandong province.Conclusion The capacity building of nucleic acid testing at all levels of dis-ease prevention and control institutions in Qingdao city is not yet balanced,the composition of personnel needs to be opti-mised,automated equipment is not sufficiently equipped,and the construction of information system needs to be strength-ened.
Centre for Disease Control and PreventionNucleic Acid TestingLaboratoryCapacity Building
NETL
NSTL
万方数据
