Research progress on detection of foodborne pathogens in viable but nonculturable state
The ability to rapidly detect live pathogens in food is crucial for public health and food safety reasons.Various factors such as food composition,storage conditions,and cooking methods can lead to certain live pathogens entering a via-ble but nonculturable state(VBNC),making traditional culture methods ineffective for detection.Detection of pathogens in the VBNC state can be achieved through induced culture methods and non-culture detection methods.Induced culture re-quires personalized induction protocols for different types of bacteria,while non-culture detection methods are diverse,in-cluding nucleic acid detection,flow cytometry,phage detection,biosensor detection,and gene sequencing.These methods have their own advantages in terms of detection speed,sensitivity,and cost.This review summarizes the latest detection methods and characteristics of pathogens in the VBNC state in recent years,discusses their limitations and future pros-pects,and provides reference for the detection of VBNC foodborne pathogens.
Foodborne pathogensViable but nonculturableVBNCDetection method
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