2020-2023年山东省潍坊市腹泻人群肠炎沙门菌分子分型及耐药性分析
Analysis on typing characteristics and drug resistance of Salmonella Enteritidis isolated from patients with diarrhea in Weifang city,Shandong province from 2020 to 2023
张凤娟 1汪忆梦 1王倩楠 1马淑青 1郑婵幸子 1李春苗2
作者信息
- 1. 潍坊市疾病预防控制中心微生物检验科,山东 261061
- 2. 山东大学附属儿童医院(济南市儿童医院)输血科
- 折叠
摘要
目的 分析2020-2023年山东省潍坊市腹泻人群中分离的肠炎沙门菌临床分离株的分子分型及抗生素耐药性,为肠炎沙门菌的预警和防控提供数据支持.方法 收集2020-2023年山东省潍坊市国家致病菌识别网哨点医院的沙门菌菌株,进行血清分型,随机选取48株肠炎沙门菌,采用脉冲场凝胶电泳(PFGE)、多位点序列分型(MLST)、基于全基因组测序(WGS)技术的单核苷酸多态性(SNP)方法进行分子分型,采用微量肉汤法对肠炎沙门菌进行17种抗生素的药物敏感性实验.结果 48株肠炎沙门菌经PFGE聚类得到24个带型,相似度为48.61％～100.00％,34个菌株形成10个簇;MLST型别均为ST 11;WGS-SNP系统发育树只可见3簇;48株菌株药物耐药率最高的为萘啶酸100.0％耐药,中介率最高的为环丙沙星(89.6％),100.0％敏感的药物有头孢他啶/阿维巴坦和替加环素;总多重耐药率为97.9％,共形成17种耐药谱,以氨苄西林(AMP)+氨苄西林-舒巴坦(AMS)+多黏菌素E(CT)+萘啶酸(NAL)+链霉素(STR)为优势耐药谱.结论 2020-2023年山东省潍坊市肠炎沙门菌的多重耐药现象比较严重,PFGE带型与耐药谱之间无明显的一致性,WGS-SNP系统发育树能更好地区分不同耐药性的菌株,甚至相同耐药性的菌株.
Abstract
Objective To investigate the antimicrobial resistance and molecular genotyping of 48 Salmonella enterica serovar Enteritidis(S.Enteritidis)clinical strains isolated from patients with diarrhea from 2020 to 2023 in Weifang city,Shandong province,so as to provide a data support for the early warning and prevention of Salmonella Enteritidis.Methods Salmonella strains were collected from the sentinel hospitals of Chinese Pathogen Identification Net form 2020 to 2023 in Weifang city of Shandong province,and serotyping was performed.Then 48 Salmonella Enteritidis strains were randomly selected to carry out drug sensitivity tests,Pulse Field Gel Electrophoresis(PFGE),Multi-locus Sequence Typ-ing(MLST),Single Nucleotide Polymorphism(SNP)based on whole genome sequencing(WGS).Drug sensitivity experi-ments was conducted on 17 antibiotics against Salmonella enterica by using the micro broth method.Results In the 48 Salmonella Enteritidis,24 PFGE patterns were detected,with similarity of 48.61％to 100％,and 34 strains of Salmonella Enteritidis formed 10 clusters.MLST was all ST11 types.There were 3 clusters on the basis of WGS-SNP phylogenetic tree.The 48 Salmonella Enteritidis strains were all resistance to nalidixic acid(100％).The intermediary rate to ciprofloxacin was highest(89.6％).The 48 Salmonella Enteritidis strains were all sensitive to ceftazidime/avibac-tam and tigecycline.The overall multi-drug resistance rate was 97.9％,showing 17 multi-drug resistance spectrums,with AMP+AMS+CT+NAL+STR as the dominant resistance spectrum.Conclusion The multidrug resistance of Salmonella Enteritidis is common in Weifang city from 2020 to 2023,and there is no obvious consistency between PFGE banding and drug resistance spectrum.WGS-SNP can better distinguish strains with different drug resistance,or even strains with the same drug resistance.
关键词
腹泻/肠炎沙门菌/分子分型/耐药性/药敏试验Key words
Diarrhea/Salmonella Enteritidis/Molecular typing/Drug resistance/Drug sensitivity testing引用本文复制引用
基金项目
山东省潍坊市卫生健康委员会项目(WFWSJK-2021-101)
出版年
2024
NETL
NSTL
万方数据