Objective To analyse the expression and prognostic significance of the never-expressed mitogen A-related ki-nase 2(NEK2)gene in malignant pleural mesothelioma(MPM).Methods The gene expression data,clinicopathological data and survival status data of MPM were downloaded from the cancer genome atlas(TCGA)database,and perl software and R language packages such as limma and timeROC were used to make differential analysis of NEK2 gene in MPM,clini-copathological correlation analysis,COX unifactorial and multifactorial analysis as well as gene set enrichment analysis,and the ROC curve was plotted;and TIMER2.0 database was used to analyze correlation between NEK2 expression in MPM and common immune cell infiltration.12 MPM tissues and 6 non MPM pleural tissues were selected,and the expression of NEK2 gene in MPM and non tumor tissues was verified by RT-qPCR.Results NEK2 was highly expressed in MPM tissues com-pared with normal lung tissues(P<0.001).mRNA expression of NEK2 gene was significantly correlated with MPM lymph node staging(P<0.05),high expression of NEK2 was correlated with poor prognosis of MPM patients(P<0.001);and the expression of NEK2 gene was positively correlated with the infiltration levels of B-lymphocytes,macrophages,and dendritic cells in MPM(P<0.05),and they were significantly enriched in pathways such as cell cycle,DNA replication,spliceo-some,mRNA monitoring,etc.In the collected case samples,compared with non MPM pleural tissue,the expression level of NEK2 gene in MPM tissue was significantly increased(P<0.01).Conclusion NEK2 is highly expressed in MPM tissues,and the high expression of NEK2 in MPM suggests a poor prognosis,which may be a potential therapeutic target for MPM.
关键词
恶性胸膜间皮瘤/永不有丝分裂基因A相关激酶2/癌症基因组图谱/预后/生物信息学
Key words
malignant pleural mesothelioma(MPM)/never-expressed mitogen A-related kinase 2(NEK2)/the cancer genome atlas(TCGA)/prognosis/bioinformatics
维普
万方数据