Inhibition of microRNA-338-3p on migration and invasion of HepG2 hepatoma cells
Objective To investigate the effects of microRNA-338-3p on migration,invasion and epithelial mesenchymal transformation of HepG2 hepatoma cells.Methods HepG2 hepatoma cells were extracted.They were randomly divided into three groups:control group(non-transfected),vector group(transfected with empty plasmid)and OE group(transfected with microRNA-338-3p plasmid).Normal saline,empty plasmid and microRNA-338-3p were transfected,respectively,and cultured for 48 hours.Real-time fluorescence quantitative PCR(qPCR)was used to detect the transfection efficiency of mi-croRNA-338-3p.The migration index of cells was detected by scratch test.The invasive ability of cells was detected by Tran-swell method.The relative protein expression levels of E-cadherin and N-cadherin were detected by Western blot assay.Results(1)The positive rate of microRNA-338-3p transfection in this study was over 90%,which met the experimental criteria.(2)The migration index of HepG2 liver cancer cells showed no statistically significant difference between the con-trol group and the vector group(P>0.05).The migration index of the OE group was significantly lower than that of the con-trol group and the vector group,and difference was statistically significant(P<0.01).(3)There was no statistically signifi-cant difference in the invasion ability of HepG2 hepatocellular carcinoma cells in the control group and the vector group(P>0.05);compared with the control group and the vector group,the invasion ability of HepG2 in the OE group significantly de-creased,and the difference was statistically significant(P<0.001).(4)There was no statistically significant difference in the relative protein expression of E-cadherin between the control group and the vector group(P>0.05);the relative protein expression of E-cadherin in the OE group was higher than that in the control group and the vector group,and the difference was statistically significant(P<0.05).(5)There was no statistically significant difference in the relative protein expression of N-cadherin between the control group and the vector group(P>0.05);compared with the control group and the vector group,the relative protein expression of N-cadherin decreased in the OE group,and the difference was statistically significant(P<0.01).Conclusion(1)microRNA-338-3p can inhibit the migration and invasion ability of HepG2 liver cancer cells;(2)microRNA-338-3p inhibits epithelial mesenchymal transition in HepG2 liver cancer cells by promoting E-cadherin pro-tein expression and inhibiting N-cadherin protein expression.
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