Expression and clinical significance of COL1A1 in cerebral gloma based on bioinformatics analysis
Objective To study the expression of COL1A1 gene in brain glioma,analyze relationship between COL1A1 gene and clinical characteristics and prognosis of patients with brain glioma,and explore the immunosuppressive target of COL1Al,so as to provide theoretical basis for future researches on immunotherapy of brain glioma.Methods The gene ex-pression profile and clinical data of brain glioma were downloaded from the official database of Chinese glioma genome atlas(CGGA)and the cancer genome atlas(TCGA).Graph Pad Prism 9 software was used to analyze the relationship between COL1A1 and brain glioma staging.R4.1.2 software and R Studio software were used to draw Pheatmap,Heatmap,Ggplot2 and Circos diagrams to analyze the relationship between COL1A1 expression and clinical information,prognosis of patients with glioma and the immunosuppressive targets.The function of COL1A1 gene was analyzed by GO and KEGG.GSVA en-richment method was used to analyze the number of gene grids interacting with COL1A1 gene.Results The expression lev-els of COL1A1 gene in WHO stage Ⅲ patients were significantly higher than those in WHO stage Ⅰ and WHO stage Ⅱ pa-tients,and differences were statistically significant(Dataset mRNAseq_325,P＜0.0001;Dataset mRNAseq_693,P=0.0018).With the increase of COL1A1 expression,the expressions of glioma specific markers such as Non-codel 1P19q,Wildtype IDH,and un-methylated MGMT were also more intensive.Heatmap survival analysis chart showed that patients with high expression of the COL1A1 gene had a lower overall survival rate than those with low expression.The results of GO analysis and KEGG analysis showed that the COL1A1 gene might exert its biological functions through extracellular regions,endoplasmic reticulum lumens,extracellular spaces,extracellular matrix structural components,collagen binding,and inte-grin binding proteins.The GSVA enrichment analysis results were consistent with the GO and KEGG analysis results,and the expression of COL1A1 gene was positively correlated with the above-mentioned gene functions.Circos plot suggested that the immune checkpoint most closely associated with glioma was the signal regulatory protein(S1RP).STRING database re-sults showed that there were a total of 10 genes interacting with COL1A1 gene.Conclusion COL1A1 gene is closely related to the stage of glioma,the prognosis of patients and the characteristics of malignant progression of the disease,and can be used as a potential molecular marker of glioma.
万方数据
维普
