摘要
目的 研究肺炎支原体肺炎(MPP)患儿肺泡灌洗液中社区获得性呼吸窘迫综合征毒素(CARDS TX)、高迁移率族蛋白 1(HMGB1)、Toll样受体 2(TLR2)表达,探讨其在MPP发病中的临床意义.方法 回顾性分析 55 例MPP病例组及 20 例对照组临床资料,同时检测两组支气管肺泡灌洗液(BALF)中CARDS TX、HMGB1、TLR2、TLR4、晚期糖基化终末产物受体(RAGE)及髓样分化因子88(MyD88)表达,体外检测不同浓度CARDS TX刺激下HMGB1、TLR2 的表达并进行比较.结果 与对照组相比,MPP 组LYM绝对值计数、PA、CD3+、CD3+CD4+、CD3+CD8+、CD3-CD19+、NK cell、CD19+CD23+明显下降,CRP、LDH、D-二聚体、IgA、IgG、IgM、CARDS TX、HMGB1、TLR2、MyD88 均升高,差异有统计学意义(P<0.05 或 0.001).CARDS TX刺激后HMGB1、TLR2 的表达升高,且呈正相关,差异有统计学意义(P<0.001).结论 CARDS TX可能通过HMGB1-TLR2-MyD88 途径参与肺炎支原体(MP)的致病.
Abstract
Objective To investigate the expressions of community-acquired respiratory distress syndrome toxin(CARDS TX),high mobility group box-1 protein(HMGB1)and Toll-like receptors 2(TLR2)in bronchoalveolar lavage fluid in chil-dren with mycoplasma pneumoniae pneumonia(MPP),and to explore the clinical significance of CARDS TX,HMGB1 and TLR2 in the pathogenesis of MPP.Methods Retrospective analysis was carried out on the clinical data of 55 MPP children and 20 children in control group.And the expressions of CARDS TX,HMGB1,TLR2,TLR4,receptor of advanced glyca-tion endproducts(RAGE)and myeloid differentiation primary response 88(MyD88)in bronchoalveolar lavage fluid(BALF)of the two groups.In addition,the expressions of HMGB1 and TLR2 after stimulation with different concentrations of CARDS TX in vitro were detected and compared.Results Compared with the control group,LYM absolute value count,PA,CD3+,CD3+CD4+,CD3+CD8+,CD3-CD19+,NK cell,and CD19+CD23+were significantly decreased,while CRP,LDH,D-dimer,IgA,IgG,IgM,CARDS TX,HMGB1,TLR2 and MyD88 were significantly increased,and difference was statistically significant(P<0.05 or 0.001).The expression of HMGB1 and TLR2 increased after CARDS TX stimulation,there was a positive correlation between them,and difference was statistically significant(P<0.001).Conclusion CARDS TX may be involved in the pathogenesis of mycoplasma pneumoniae(MP)through HMGB1-TLR2-MyD88 pathway.
基金项目
苏州市临床重点病种诊疗技术专项拟定项目(2023)(LCZX202313)
维普
万方数据