Analysis on drug resistance phenotype and genotype characteristics of 36 carbapenem resistant Klebsiella pneumoniae strains
Objective To analyze the drug resistance and drug resistance mechanism of 36 strains of carbapenem-resist-ant Klebsiella pneumoniae(CRKP)isolated in a national comprehensive three A-level hospital from 2020 to 2022 in Baise,Guangxi,so as to provide basis for hospital infection prevention and control and the guidance of clinical treatment.Methods The resistance of 36 strains of CRKP to common clinical antibiotics and ceftazidime/avertam was detected by broth mi-crodilution method and Kirby-Bauer method.At the same time,polymerase chain reaction was used to detect the carrying of common drug resistance genes.Carbapenem enzyme inhibitor enhancement test was used to detect the types of carbapenem enzymes carried by the strains.Results 36 CRKP showed over 90% resistance to all β lactaams and fluoroquinolones ex-cept ceftazidime/avibactam,33.3% resistance to ceftazidime/avibactam,63.9% resistance to amikacin,and 61.1% resist-ance to sulfa cotrimoxazole.The carrying rate of blaKPC,blaNDM,blaTEM,blaCTX-m,blaSHV,and blaAmpc was 63.89%,19.44%,75.00%,16.67%,91.67%,and 5.56%,respectively.No strain carrying blaOXA-48 was detected.Phenotypic de-tection showed that 34 strains produced carbapenem,of which 23 strains produced class A enzymes,10 strains produced class B enzymes,and 1 strain produced both class A and B enzymes.Conclusion Investigation of common CRKP resist-ance and gene phenotypes can provide a scientific basis for the rational application of clinical antibiotics and the prevention and control of infection.
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