Objective To detect Klebsiella pneumoniae carbapenemase(KPC)producing carbapenem-resistant Klebsiella pneumoniae(CRKP)by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and to screen and verify its characteristic peak,so as to establish a suitable method for rapid detection of KPC-producing CRKP in our laboratory.Methods 39 clinical strains of CRKP were collected,and 23 of them were detected to produce KPC by PCR.MALDI-TOF MS technology was used to detect KPC-positive CRKP strains,Flex Analyst software was used to analyze and screen out their characteristic peaks after the mass spectrum was obtained,and then repeatable experiments and clinical verification evaluations were carried out.Results The best characteristic peak of KPC-producing CRKP was m/z 6432.The specificity and sensitivity of the validation experiment and the repeatability experiment of characteristic peak were 96.67%and 100%,respectively.The specificity and sensitivity of clinical validation evaluation were 92.31%and 100%,respective-ly.Conclusion The best characteristic peak of KPC-producing CRKP that can be detected by using MALDI-TOF MS tech-nology is m/z 6432.It is proved by experiments that this characteristic peak has high specificity and sensitivity,which can provide a theoretical basis for rapid clinical diagnosis and treatment of KPC-producing CRKP.
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