Expression levels and significance of GDNF,GAP-43,NSE,and S-100 proteins in children with Hirscbsprung's disease
Objective To explore and analyze the expression levels and significance of glial cell derived neurotrophic fac-tor ( GDNF),growth-associated protein-43 ( GAP-43),neuron-specific enolase ( NSE),and S-100 protein in children with Hirschsprung's disease (HD).Methods 72 children with HD who were diagnosed and treated from September 2019 to Oc-tober 2022 were selected as HD group,and 72 children who underwent colon surgery for other non intestinal ganglion lesions during the same period were selected as control group.Colon full-thickness pathological specimens of the two groups were taken,and immunohistochemical analysis of GDNF,GAP-43,NSE,and S-100 protein expressions was performed.At the same time,diagnostic scoring and correlation analysis for Hirschsprungs-associated enterocolitis ( HAEC) were conducted,and tissue samples from different intestinal segments of the HD group were collected for testing.Results The positive ex-pression rates of GDNF,GAP-43,NSE,and S-100 proteins in the full-thickness colonic specimens of the HD group were 77.8％,73.6％,81.9％,and 83.3％,respectively,which were significantly higher than those in the control group (22.2％,26.4％,20.8％,and 22.2％,respectively) (P<0.001).There were statistically significant differences in the positive expression rates of GDNF,GAP-43,NSE,and S-100 proteins in different colonic regions ( narrow segment,transi-tional segment,dilated segment,and normal segment) of children with HD ( P<0.001).The HAEC diagnostic score of the HD group was significantly improved compared to that of the control group ( P<0.001).In the HD group,Spearman analysis showed that there was a positive correlation between the HAEC diagnostic score and the positive expression rates of GDNF,GAP-43,NSE,and S-100 proteins in the full-thickness of the colon (P<0.001).Conclusion Children with HD are most-ly accompanied by high expressions of GDNF,GAP-43,NSE and S-100 protein,which is related with the condition of chil-dren and deserves clinical attention.
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