摘要
目的 对一株从乙肝肝硬化患者血液中分离的病原菌进行鉴定.方法 采用标准的霍乱弧菌鉴定方法对该病原菌的培养特性、形态学特征、生化鉴定、蛋白质谱分析、血清学鉴定及荧光PCR进行分析.结果 根据乙肝肝硬化患者血液中分离的菌株培养特性、形态学特征、生化鉴定、蛋白质谱分析(在4279m/z、4367m/z、5123m/z、6165m/z、6277m/z、6453m/z和7160m/z采集到特征性质谱峰)、血清学鉴定及荧光PCR(霍乱弧菌O1群和O139群基因均为阴性)鉴定为非O1/非O139群霍乱弧菌.结论 广西百色地区首次在乙肝肝硬化患者血液中分离出非O1/非O139群霍乱弧菌.
Abstract
Objective To identify a pathogen isolated from the blood of a patient with hepatitis B cirrhosis.Methods Standard methods for identifying vibrio cholerae were used to analyze the culture characteristics,morphological characteris-tics,biochemical identification,protein mass spectrometry analysis,serological identification,and fluorescence PCR of the pathogen.Results According to the culture characteristics,morphological characteristics,biochemical identification,pro-tein mass spectrometry analysis ( characteristic spectrum peaks were collected at 4279 m/z,4367 m/z,5123 m/z,6165 m/z,6277 m/z,6453 m/z and 7160 m/z),serological identification and fluorescent PCR (vibrio cholerae O1 and O139 genes were negative) of strains isolated from the blood of the patient with hepatitis B cirrhosis,they were identified as non O1/non O139 vibrio cholerae.Conclusion Vibrio cholerae non O1/non O139 is isolated from the blood of patients with hepatitis B cirrhosis in Baise,Guangxi for the first time.
维普
万方数据