Research on the protective effect and mechanism of kaempferol on oxidative damage and apoptosis of human lens epithelial cells induced by H2O2
Objective To investigate the effects of kaempferol on the oxidative damage and apoptosis of human lens epithelial cells induced by hydrogen peroxide(H2O2),as well as its regulatory role in the Janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3)signaling pathway.Methods Human lens epithelial cells HLE-B3 were cultured in vitro and divided into the blank group(no intervention),the model group(400 pmol•L-1 H2O2),the kaempferol group(400 μmol•L-1 H2O2+40 pmol•L-1 kaempferol),the AG490 group(400 pmol•L-1 H2O2+50 μmol•L-1 JAK2/STAT3 signaling pathway inhibitor AG490),the inhibitor group(400 pmol•L-1 H2O2+40 pmol•L-1 kaempferol+50 μmol•L-1 AG490),and the activator group(400 pmol•L-1 H2O2+40 pmol•L-1 kaempferol+0.5 μmol•L-1 JAK2/STAT3 signaling pathway activator C-A1).After 24 h of intervention,Hoechst 33258 staining was used to determine the ap-optosis rate of these cells.Malondialdehyde(MDA)and superoxide dismutase(SOD)kits were used to detect the levels of MDA and SOD.The protein expression levels of Cysteine aspartic protease 3(Caspase-3),B-cell lymphoma-2(Bcl-2),JAK2,STAT3,p-JAK2,and p-STAT3 were detected by Western blot.Results Compared with the blank group,the apop-tosis rate,the MDA level,and the protein expression levels of Caspase-3,p-JAK2,and p-STAT3 increased(all P＜0.05)in the model group,while the protein expression levels of SOD and Bcl-2 decreased(all P＜0.05).Compared with the model group,the apoptosis rate,the MDA level,and the protein expression levels of Caspase-3,p-JAK2,and p-STAT3 decreased in the kaempferol and AG490 groups(all P＜0.05),while the protein expression levels of SOD and Bcl-2 increased(all P＜0.05).Compared with the kaempferol group,the apoptosis rate,the MDA level,and the protein expression levels of Caspase-3,p-JAK2,and p-STAT3 decreased in the inhibitor group,while the protein expression levels of SOD and Bcl-2 in-creased(all P＜0.05);the changes in the above indicators in the activator group were opposite to those in the inhibitor group(all P＜0.05).Conclusion Kaempferol can protect HLE-B3 cells from H2O2-induced oxidative damage and inhibit their apoptosis by inhibiting JAK2/STAT3 signaling pathway.
cataractlens epithelial cellskaempferolhydrogen peroxideJanus kinase 2/signal transducer and acti-vator of transcription 3(JAK2/STAT3)signaling pathwayoxidative damageapoptosis
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