摘要
目的 测量成年食蟹猴视网膜结构和功能参数,探讨非人灵长类动物与正常人视网膜结构和功能参数的相似度.方法 对3只5岁龄成年食蟹猴的6只眼进行彩色眼底照相、视网膜光学相干断层扫描(OCT)、视网膜电图(ERG)等在体的眼科学检查,测定猴眼视网膜黄斑中心凹区及距黄斑中心凹鼻侧、颞侧、上方、下方各1 000 μm、2 000 μm处的内外层视网膜厚度、视网膜神经纤维层(RNFL)厚度以及视盘面积、视杯面积、杯盘面积比等视盘参数和闪光ERG的生物学参数.比较不同眼别间各参数差异.参照既往已发表文献,比较各参数与正常人的相似度.结果 正常成年食蟹猴的视网膜黄斑中心凹处厚度为(252.31± 4.79)μm,视盘面积为(1.89±0.05)mm2,杯盘面积比为 0.14±0.01,RNFL 平均厚度为(103.53±0.58)μm.暗适应 0.01 ERG b 波振幅为(66.75±7.29)μV,暗适应 3.0 ERG a、b 波振幅分别为(57.15±15.01)、(122.10± 25.51)μV,暗适应10.0 ERG a、b波振幅分别为(72.98±20.14)和(131.67±13.78)μV,振荡电位振幅为(49.98±10.08)μV,峰时为(30.02±5.76)ms.明适应 3.0 ERG a、b 波振幅分别为(9.16±2.75)和(40.43±5.57)μV.明适应闪烁光反应峰时为(26.61±1.19)ms,振幅为(24.72±5.10)μV.左、右眼各参数比较差异均无统计学意义(均P＞0.05).食蟹猴中心凹区视网膜厚度、平均RNFL厚度、ERG的波形和振幅等结果与正常人的视网膜参数相近.结论 成年食蟹猴视网膜结构和功能与正常人相近,其作为临床前药物研究的实验动物,可参考健康人群的视网膜参数.
Abstract
Objective To measure the retinal structural and functional parameters of adult Macaca fascicularis,and explore the similarity of the retinal structural and functional parameters between non-human primates and normal human retinas.Methods Six eyes of 3 5-year-old adult Macaca fascicularis were examined by in vivo detection including color fundus photography,retinal optical coherence tomography(OCT)and electroretinogram(ERG)to determine the thickness of the inner/outer retina at the fovea and 1 000/2 000 μtm away from the nasal,temporal,superior and inferior regions of the fovea,the thickness of the retinal nerve fiber layer(RNFL),the area of optic disc,the area of optic cup,the area ratio of cup to disc and the biological parameters of flash ERG.Differences in the above parameters between left and right eyes were analyzed.The similarity of parameters between Macaca fascicularis and human was compared with reference to published literature.The use and care of animals complied with the Regulation on the Management of Experimental Animals.The study protocol was approved by the Institutional Animal Care and Use Committee of Hubei Topgene Biotechnology(NO.IACUC-2019-012).Results The foveal thickness,optic disc area,cup-disc area ratio,and average RNFL thickness in normal adult Macaca fascicularis were(252.31±4.79)μm,(1.89±0.05)mm2,0.14±0.01,and(103.53±0.58)μm,respectively.The b-wave amplitude of dark-adapted 0.01 ERG was(66.75±7.29)μV.The a-and b-wave amplitudes of dark-adapted 3.0 ERG response were(57.15±15.01)and(122.10±25.51)μV,respectively.The a-and b-wave amplitudes,the amplitude of oscillation potentials,and the latency of dark-adapted 10.0 ERG response were(72.98±20.14)μV,(131.67± 13.78)μV,(49.98±10.08)μV,and(30.02±5.76)ms,respectively.The a-and b-wave amplitudes of light-adapted 3.0 ERG were(9.16±2.75)and(40.43±5.57)μV,respectively.The latency and the amplitude of the light-adapted 30 Hz flicker was(26.61±1.19)ms and(24.72±5.10)μV,respectively.There was no significant difference in the parameters between left and right eyes(all at P＞0.05).The retinal thickness in central fovea,mean RNFL thickness,waveform and amplitude of ERG of Macaca fascicularis were similar to normal human.Conclusions The structure and function of the retina of adult Macaca fascicularis are similar to those of normal humans.As a laboratory animal for preclinical drug research,in vivo studies of Macaca fascicularis can refer to normal human retinal parameters.
NETL
NSTL
维普
万方数据