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有效降解农膜的真菌筛选与复合菌群的构建

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为解决农用地膜在土壤中难降解的问题,从土壤中筛选菌株且对不同农膜材料降解的研究.本研究从黑色聚乙烯(PE)地膜、白色(PE)地膜、聚乙烯和聚己二酸/对苯二甲酸丁二酯(PBAT)降解地膜、聚甲基乙撑碳酸酯(PPC)降解地膜等4 种不同材料覆盖下的土壤中分离筛选两株有效降解农膜的真菌,经过鉴定得到菌株Penicillium citrinum(JW1)和Aspergillus costaricensis(JW2).将JW1 和JW2菌株进行复合,观察农膜降解前后表面微观特征的改变情况.结果表明,四种覆膜材料在同时接种JW1、JW2 菌株后,均出现不同程度的降解,在接种复合菌群降解 30 d后,PPC降解地膜、PBAT降解地膜、黑色PE地膜和透明PE地膜的失重率分别为 23.78%、14.73%、22.78%和 17.55%,接种复合菌群对PPC地膜的降解效果显著高于其它三种地膜(P<0.05).本试验旨在筛选出有效降解农膜的真菌菌株,以期为农膜的微生物降解技术提供技术支持.
Screening of Fungi for Effective Degradation of Agricultural Film and Construction of Composite Microbiota
In order to solve the problem of difficult degradation of agricultural film in soil,strains were screened from soil and the degradation of different agricultural film materials was studied.In this study,black polyethylene(PE)film,white PE film,polyethylene and poly(butylene adipate/terephthalate)(PBAT)degradation film,poly(methyl ethyl carbonate)(PPC)degradation film and other four different materials were used to screen two strains of fungi that effectively degrade agricultural film.The strains Penicillium citrinum(JW1)and Aspergillus costaricensis(JW2)were identified.The JW1 and JW2 strains were combined,and the changes of surface micro-characteristics were observed before and after degradation.The results showed that the four kinds of biofilm materi-als showed different degrees of degradation after inoculation with JW1 and JW2 strains at the same time.30 days af-ter inoculation,the weight loss rates of PPC-degraded film,PBAT-degraded film,black PE film and transparent PE film were 23.78%,14.73%,22.78%and 17.55%,respectively.The degradation effect of PPC-degrading mulch by inoculation with composite bacteria was significantly higher than that of the other three mulches(P<0.05).In this study,fungal strains JW1 and JW2 were screened for effective degradation of agricultural film,so as to provide technical support for microbial degradation technology of agricultural film.

polyethylenePBATagricultural film degradationfungi

刘梦圆、王雯、刘春虎、赵殿峰

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榆林学院 生命科学学院,陕西 榆林 719000

榆林市种业工作站,陕西 榆林 719000

聚乙烯 PBAT 农膜降解 真菌

国家自然科学基金陕西省自然科学基础研究计划

417630112024JC-YBMS-205

2024

榆林学院学报
榆林学院

榆林学院学报

影响因子:0.19
ISSN:1008-3871
年,卷(期):2024.34(2)
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