Cloning and Bioinformatics Analysis of the PlHDR Gene from Paeonia lactiflora
To predict the function of the 4-hydroxy-3-methylbut-2-enyl diphosphate reductase(PlHDR)gene in Paeonia lactiflora,the PlHDR gene was cloned from Paeonia lactiflora leaves using RT-PCR technology and sub-jected to sequence analysis.The physical and chemical properties and structural characteristics of PlHDR were pre-dicted using bioinformatics software.The results showed that the full-length cDNA of the PlHDR gene from Paeonia lactiflora was 1559 bp,encoding 462 amino acids with a molecular weight of 52.11 kDa and an isoelectric point of 5.46.The PlHDR from Paeonia lactiflora was most closely related to the HDR from Camptotheca acuminata,with an amino acid similarity of 87.45%.PlHDR is a hydrophilic protein located in the chloroplast,lacking transmem-brane domains and signal peptides,containing 5 glycosylation sites and 50 phosphorylation sites.In the secondary structure,α-helix and random coil accounted for 48.48%and 29.22%,respectively.The 3D structure prediction indicated a monomer.PlHDR interacts with proteins such as HDS,DXR,CDPMEK,MK,and MVD1.This study reports the sequence of the PlHDR gene from Paeonia lactiflora and conducts bioinformatics analysis,laying the foundation for further functional studies of this gene in the synthesis of terpenoids in Paeonia lactiflora.
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