The Influence of Whole Blood Mixing Method on the Detection Results of Glycated Hemoglobin
Objective To investigate the influence of different mixing methods on the detection results of glycosylated hemoglobin(GHb)before detection.Methods With the collection of 203 venous whole blood samples from clinical patients who underwent GHb testing in the hospital from January to April 2023,the mixed and non-mixed injection methods were used for pre-test samples,and the levels of GHb subtype HbA1c were detected separately as GHb detection results.Patients were grouped according to age(<60 years old,≥60 years old)and hemoglobin level(≥130 g/L for males and≥115 g/L for females as normal,otherwise as anemia),the influence of whole blood mixing method on test results was analyzed,and the bias of test results between the two methods was calculated.Results The mixed test results of patients with different ages and hemoglobin levels were higher than the unmixed test results,and the differences were statistically significant(P<0.05).The unmixed test results showed negative bias compared to the mixed test results,and the bias between the 26 test results exceeded 1/3 of the allowable total error(6%).Conclusion Before testing HbA1c,the sample should be thoroughly mixed,and attention should be paid to the alarm information on the instrument result page.If necessary,blood collection and retesting should be conducted again or pre-dilution method should be used for retesting.
万方数据
维普
