Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of six components in Ganfu granules,including geniposide,crocin Ⅰ,tanshinone Ⅰ,cryptotanshinone,polygonatum cuspidatum glycoside,and tanshinoneⅡA.Methods Used a COSMOSILC18 chromatographic column,with acetonitrile 0.1%phosphoric acid as the mobile phase and a flow rate of 0.8 ml/min,for gradient elution with a detection wavelength of 205 nm.Results Six components all achieved good separation,and within a certain concentration range,each component showed a good linear relationship.The average recovery rate was from 97.92%to 99.67%,and the RSD was from 0.28%to 1.51%.Quantitative data was analyzed by statistical software,and it was found that geniposide and crocinⅠ were the main factors affecting the differences in batch samples.Conclusion Establishing a high-performance liquid chromatography(HPLC)method to simultaneously determine the content of six components in Ganfu granules,including geniposide,crocinⅠ,tanshinoneⅠ,cryptotanshinone,polygonatum cuspidatum glycoside,and tanshinoneⅡA,is beneficial for the quality control of Ganfu granules.
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