Analysis of the Value of Serum AFP-L2 and NIPT in and NT Screening Fetuses
Objective To explore the application value of serum AFP-L2 and NIPT technology combined with ultrasound NT value for the screening of 24～28+6 fetuses.Methods A total of 120 pregnant women who received early pregnancy examination to confirm chromosomal abnormalities in hospital from September 2021 to September 2022 were selected as the abnormal chromosome group,and 120 pregnant women who underwent normal pregnancy examination in hospital and had completed screening for fetal chromosomal abnormalities were selected as healthy volunteers for case-control study.The ultrasound examination NT,serum AFP-L2 and NIPT technology were tested together for all the pregnant women.AFP-L2 levels were detected by enzyme-linked free adsorption assay.Statistical comparison of positive and negative detection of the four detection methods improves the diagnostic efficiency of screening for the four fetal chromosome abnormalities.Results With lower AFP-L2 levels and higher NT thickness(P<0.05).Compared with ultrasound,positive,positive and negative predictive value(P<0.05),positive and negative predictive value,positive and NIPT,combined examination,positive and negative,positive and negative predictive value(P<0.05).Compared with sonography,NIPT,combined examination showed high sensitivity,specificity and accuracy(P<0.05),the NIPT total positive value(positive predictive value,PPV)was 92.24%;For 54,X(included chimeras),54,XXX,54,XXY,54,XYY,and sex chromosome microdeletion,the PPV were 49.05%,79.15%,85.55%,43.19%,and 57.69%,respectively.The ultrasound test has lower sensitivity,specificity,and accuracy,NIPT,specificity,with statistical difference(P<0.05).Conclusions Screening of fetal chromosomal abnormalities through ultrasound examination,serum AFP-L2 and NIPT technology can improve the accuracy of screening for fetal chromosomal abnormalities,reduce the misdiagnosis rate,and have good diagnostic efficacy.The combination of ultrasound and serum with NIPT is important for screening fetal chromosomal abnormalities.
NETL
NSTL
万方数据
