Application of Nucleic acid Testing and Colloidal Gold Immunochromatography in the Diagnosis of Mycoplasma Pneumoniae Infection in Children
Objective To investigate the diagnostic value of Mycoplasma pneumoniae nucleic acid detection(MP-DNA,MP-RNA)and Mycoplasma pneumoniae antibody measurement(MP-IgM)for Mycoplasma pneumoniae infection in children.Methods Peripheral blood specimens and pharyngeal swabs of 201 children with respiratory diseases admitted to the hospital from November 2023 to February 2024 were collected,and MP-RNA was detected by fluorescent nucleic acid thermostatic amplification detection,pharyngeal swab specimens were taken from-70℃frozen storage,and MP-DNA was detected by real-time fluorescence quantitative PCR,and MP-IgM was detected by colloidal gold immunochromatography,respectively.-DNA and MP-IgM were compared with the gold standard(MP-RNA),and the consistency of the results was statistically determined.Results The sensitivity of MP-DNA was 89.47%,the specificity was 97.55%,and the concordance with MP-RNA results was excellent(Kappa=0.87).The sensitivity of MP-IgM was 34.21%,the specificity was 93.87%,and the concordance with MP-RNA results was poor(Kappa=0.33).Conclusion MP-DNA has higher application value in the diagnosis of Mycoplasma pneumoniae compared with MP-IgM,especially in the early stage of infection.For meeting the demand for timely and efficient laboratory reporting in outpatient and emergency clinics,the combination of antibody assay and nucleic acid detection method can be considered to improve the diagnostic ability of the laboratory for Mycoplasma pneumoniae.
Mycoplasma pneumoniaeChildrenNucleic acid testImmunoglobulin M
万方数据
维普
